Project description:We used microarrays to determine the effect of prenatal nicotine exposure on gene expression profiles in the striatum of adolescent rats. We found a number of immediate early genes to be differentially expressed due to food-restriction.
Project description:We used microarrays to determine the effect of prenatal nicotine exposure on gene expression profiles in the striatum of adolescent rats. We found a number of immediate early genes to be differentially expressed due to food-restriction. We compared control (n=8), nicotine exposed (n=7) and a group of animals matched to the food intake of nicotine exposed animals (n=10) to identify gene expression changes associated with prenatal nicotine exposure
Project description:The aim of the study was to investigate the involvement of the mTOR pathway in the autism spectrum disorder. Adolescent male rats, prenatally exposed to valproic acid (500 mg/kg ip on gestational day 12.5), were treated with the mTOR inhibitor rapamycin (10 mg/kg, ip, two consecutive days starting at postnatal day 35). Transcriptomic analysis was performed on the ventral portion of coronal sections, containing the striatum. Behavioral experiments, the electrophysiology on striatal slices, a morphological analysis by confocal microscopy and the determination synaptic proteins expression in the nucleus accumbens were also performed.
Project description:Formaldehyde (HCHO) is the simplest form of aldehyde and it is naturally present in a wide range of resources. In spite of its cosmopolitan presence, formaldehyde can have deleterious health effects at higher concentrations like leukemia. However, most of the studies carried out so far have focused on the effect of formaldehyde exposure through inhalation and not much has been studied on the its exposure through food. In this context, the present study was carried out to investigate the effect of formaldehyde exposure through drinking water on the liver proteome of rat which would not only be helpful in assessing the impact of formaldehyde on health of organisms but also would be helpful in understanding the mechanism of detoxification.
Project description:Banned from mass production, polychlorinated biphenyls (PCBs) still pose a neurological health threat decades later. PCB52, a volatile compound, has been found in the indoor air of schools where adolescent students and staff are exposed. The adolescent brain is vulnerable and highly influenced by the environment around it. This study used adolescent Sprague-Dawley rats to determine the neurological effects of a subacute (28-day) PCB52 inhalation exposure. Rats were exposed 4 h a day for 28 consecutive days with neurobehavioral tests conducted during the last 5 days of exposure. Based on the data including brain weights, PCB52 and metabolite levels, gene expression in the striatum and cerebellum, and neurobehavioral tests (Y-maze, HBT, OFT, and EPM); a subacute inhalation of PCB52 results in sex-specific differences in response to exposure. Our results indicate multilevel PCB52 influence that is different in adolescent males and females; from gene expression to downstream innate mechanisms driving behavior.
Project description:Male Sprague-Dawley rats were used to establish exhausted-exercise model by motorized rodent treadmill. Yu-Ping-Feng-San at doses of 2.18 g/kg was administrated by gavage before exercise training for 10 consecutive days. Quantitative proteomics was performed for assessing the related mechanism of Yu-Ping-Feng-San.
