Project description:This RNA-Seq analysis compares gene expression of the wild-type fission yeast (Schizosaccharomyces pombe) strain at various times after transfer to defined phosphate-replete ePMGT(+PO4) medium (2, 4, 8 HR) contrasting to the WT fission yeast S. pombe cells grown in phosphate-replete YES medium (0HR).
Project description:Arsenic is known as a human carcinogen that easily be exposed by the living organisms through environment and food consumption. The arsenic is transport into the cells via phosphate transporters due to its structural similarity with phosphate in both prokaryotes and eukaryotes. We here evaluated and analyzed the toxicogenomic impacts of arsenate and the role of different phosphate concentrations on arsenic toxicity. Our results showed that arsenic uncoupled phosphate levels which eventually affected the growth rate of yeast cells. Analysis of arsenate levels in the medium over 4 to 10 h of its exposure clearly showed that arsenate was easily taken up by the cells in phosphate limited condition.
Project description:The project quantified the yeast phosphoproteome before and at various times after dithiolthreitol (DTT) in budding yeast, focusing on wild-type cells and mutants laking the Ire1 and Mkk1/Mkk2 kinases.
Project description:Diploid wild type cells in a chemostat, in different times after changing the feeding medium from low to high Pi. Diploid cells were also grown in a chemostat at a dilution rate of 0.3[1/hr] and phosphate concentration in the feeding vessel of 0.3mM Pi. Cells were allowed to reach steady state and then feeding medium was replaced to 7.3mM Pi. Samples from the chemostat were harvested in different times after the transfer (t= 0.35, 2, 5, and 10 days). Total RNA was extracted using MasterPure™ Kit (Epicentre). The samples were amplified, labeled, hybridized to yeast dual color expression microarrays and scanned, all using standard Agilent protocols, reagents, and instruments. The scanned images were analyzed using SpotReader software (Niles Scientific).
Project description:Investigation of Saccharomyces cerevisiae phosphate metabolism. Cells starved for phosphate, cells grown with intermediate and high phosphate concentrations, and PHO4 mutant cells examined. Keywords: other
