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Drosophila melanogaster

ABSTRACT: CTCF/CP190 and ISWI dependent regulation of nucleosome occupancy [ChIP-seq]

PROVIDER: PRJNA223674 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1015601.fastq.gz	Fastqsanger.gz
	SRR1015602.fastq.gz	Fastqsanger.gz
	SRR1015603.fastq.gz	Fastqsanger.gz

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Drosophila melanogaster

Project description:CTCF/CP190 and ISWI dependent regulation of nucleosome occupancy [MNase-seq]

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Project description:H3-ChIP-seq was performed in order to analyze changes in nucleosomal occupancy after depletion of CTCF/P190 and ISWI from Drosophila S2 cells Histone H3 ChIP-seq from Drosophila S2 cells after CTCF/CP190 or ISWI-specific RNAi treatment

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Analysis of genome-wide binding of the insulator factors CTCF and CP190

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Project description:CCCTC-binding factor (CTCF) is a conserved protein able to block communication between regulatory elements and gene promoters in flies and mammals. Here, we studied CTCF function in the Drosophila central nervous system (CNS) in which we find CTCF plays a vital role. Chromatin immunoprecipitation (ChIP)-sequencing of CTCF and its partner protein Centrosomal protein 190 kDa (Cp190) in CNSs of wildtype (WT), CTCF[0] mutants completely lacking CTCF and Cp190[KO] mutants with a CRISPR/Cas9-mediated deletion of the Cp190 open reading frame identified CTCF and Cp190 peaks defined as enriched in WT relative to each respective mutant control. These experiments also revealed that CTCF recruits Cp190 to CTCF's binding sites, but Cp190 is dispensable for CTCF binding to most of CTCF's sites.

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