Project description:This is a mathematical model describing the hematopoietic lineages with leukemia lineages, as controlled by end-product negative feedback inhibition. Variables include hematopoietic stem cells, progenitor cells, terminally differentiated HSCs, leukemia stem cells, and terminally differentiated leukemia stem cells.
Project description:RNA expression analysis on purified human long-term and short-term hematopoietic stem cells (LT-HSC, ST-HSC), common myeloid and megakaryocyte-erythrocyte progenitor cells (CMP, MEP) using microarrays. FACS-purified hematopoietic stem and progenitor cell (HSPC) subsets were analyzed for changes in RNA expression using NimbleGen gene expression microarrays. Analysis of RNA expression of bone marrow-derived HSPC subsets of healthy human donors.
Project description:RNA expression analysis on purified human long-term and short-term hematopoietic stem cells (LT-HSC, ST-HSC), common myeloid and megakaryocyte-erythrocyte progenitor cells (CMP, MEP) using microarrays. FACS-purified hematopoietic stem and progenitor cell (HSPC) subsets were analyzed for changes in RNA expression using NimbleGen gene expression microarrays.
Project description:Transplantation of human hematopoietic stem cells into immunodeficient mice is a valuable technique to gain insight into stemness and hematopoietic development in an in vivo environment. In this study we analyzed if transplanted hematopoietic cells in humanized mice recapitulate epigenetic changes of normal hematopoietic development. Hematopoietic stem and progenitor cells (CD34+) were isolated from human umbilical cord blood (CB) and 50,000 cells were intravenously injected into five 7 to 9 week old KIT-deficient NOD/SCID Il2rg-/-KitW41/W41 (NSGW41). 19 weeks after transplantation bone marrow was harvested, sorted for hCD45+ cells, and DNA methylation profiles were analyzed with Infinium HumanMethylation450 BeadChips (Illumina). DNA methylation patterns of normal hematopoietic development are overall recapitulated in the xenogeneic microenvironment – particularly those of normal early B cell progenitor cells.
Project description:DNA methylation analysis on purified human long-term and short-term hematopoietic stem cells (LT-HSC, ST-HSC), common myeloid and megakaryocyte-erythrocyte progenitor cells (CMP, MEP) using HELP arrays. FACS-purified hematopoietic stem and progenitor cell (HSPC) subsets were analyzed for changes in DNA methylation using NimbleGen HELP microarrays. Analysis of DNA methylation of bone marrow-derived HSPC subsets of healthy human donors.
