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Comparative total RNA and mRNA sequencing and systems analysis reveals nascent transcriptional response to early HIV-1 infection in a CD4+ T cell line

ABSTRACT: Comparative total RNA and mRNA sequencing and systems analysis reveals nascent transcriptional response to early HIV-1 infection in a CD4+ T cell line

OTHER RELATED OMICS DATASETS IN: GSE53993

PROVIDER: PRJNA234297 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1106190_1.fastq.gz	Fastqsanger.gz
	SRR1106190_2.fastq.gz	Fastqsanger.gz
	SRR1106200_1.fastq.gz	Fastqsanger.gz
	SRR1106200_2.fastq.gz	Fastqsanger.gz
	SRR1106191_1.fastq.gz	Fastqsanger.gz

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Comparative total RNA and mRNA sequencing and systems analysis reveals nascent transcriptional response to early HIV-1 infection in a CD4+ T cell line

Project description:To systematically investigate early host transcriptional response to HIV-1 infection, including polyadenylated and non-polyadenylated transcripts, we separately sequenced Total RNAs (Total RNA-seq) from HIV-1-infected SupT1 cells, a human CD4+ T cell line. We show many non-polyadenylated RNAs were also differentially expressed upon HIV-1 infection, as expected only detected by Total RNA-seq. Interestingly, we identified 8 times more differentially expressed genes at 12 hour post-infection by Total RNA-seq than by mRNA-seq, mostly protein-coding genes.

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2017-02-28 | PXD005234 | Pride

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