Project description:Total RNA of KSHV-infected cells were extracted and RT-PCR was performed using viral circRNA specific divergent primers. Amplicons were sequenced with MiSeq.
Project description:For each sample a matched total was produced in parallel. Amplicons were made from each and hybridized on human 5.0 kb promoter arrays from Nimblegen. Keywords: ChIP-chip
Project description:Chromatin immunoprecipitation of the MCM2-7 replicative helicase followed by hybridization to genome-wide tiling microarrays demonstrated that MCMs localize to the follicle cell amplicons at stages of replication initiation
Project description:Chromatin immunoprecipitation of RNAPII followed by hybridization to genome-wide tiling microarrays demonstrated that RNAPII localizes to all six Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified genomic loci
