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Mus musculus

ABSTRACT: RNA-seq profiling of transcriptomes of control and Hif1a mutant E12.5 hearts

PROVIDER: PRJNA260617 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1571970_1.fastq.gz	Fastqsanger.gz
	SRR1571970_2.fastq.gz	Fastqsanger.gz
	SRR1571971_1.fastq.gz	Fastqsanger.gz
	SRR1571971_2.fastq.gz	Fastqsanger.gz
	SRR1571972_1.fastq.gz	Fastqsanger.gz

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ChIP-seq profiling of loci bound by HIF1a in wild-type E12.5 hearts

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RNA-seq profiling of transcriptomes of control and Hif1a mutant E12.5 hearts

Project description:Purpose: to identify genes aberrantly expressed upon myocardial ablation of Hif1a Methods: a floxed Hif1a allele was deleted in mouse embryonic hearts using a NXK2.5Cre line. Total RNA was extracted from E12.5 hearts (n=3 for controls and mutants) usinz Trizol and processed for RNA-seq. Reads were mapped to Mm10 reference genome using TopHat2 and Bowtie2. Transcript expression values were determined after transcript normalization with AltAnalyze Results: this analysis revealed a total of 1451 genes significantely (|Fold| > 20% and P<0.05) modulated in Hif1a cKO hearts

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The Gene Expression Analysis in E12.5 Mouse Hearts with GATA4-FOG2 Interaction Loss

Project description:In order to identify the targets of GATA4-FOG2 action in mammalian heart development we performed Affymetrix microarray comparisons of gene expression in normal and mutant at embryonic (E) day E12.5 hearts. We compared RNA samples from both Fog2-null and Gata4ki/ki mutant E12.5 hearts to the wild-type control E12.5 hearts. We reasoned that as the phenotypes of the Fog2 knockout and Gata4ki/ki mutation (a V217G mutation that specifically cripples the interaction between GATA4 and FOG proteins) are similar, we should expect to identify a similar set of differentially expressed genes in both experiments. As an additional control, we expected to find the Fog2 gene expression absent in the mutant (null) Fog2 cardiac sample, but not Gata4ki/ki sample.

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