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Bothrops jararaca

ABSTRACT: Comparative transcriptomics of tissues from the snake Bothrops jararaca

PROVIDER: PRJNA262899 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1596200.fastq.gz	Fastqsanger.gz
	SRR1596201.fastq.gz	Fastqsanger.gz
	SRR1596202.fastq.gz	Fastqsanger.gz
	SRR1596063.fastq.gz	Fastqsanger.gz
	SRR1596064.fastq.gz	Fastqsanger.gz

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Bothrops jararaca

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Increase of cancer-related proteins abundance upon Bothrops jararaca snake venom treatment of breast cancer cell lines

Project description:In this work of Kisaki et al, we analyze the variation of proteome responses upon treatment of breast cancer cell lines MCF7 and MDA-MB231 with Bothrops jararaca snake venom

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Qualitative and quantitative analysis of Bothrops jararaca venom gland proteome

Project description:While cellular transcripts encode rich information that provide key features to understand the molecular basis of snake venom variation, their presence/ abundance does not necessary imply/correlate in the translation of a functional (protein) product. In this study we carried out an analysis of the venom gland proteome of Bothrops jararaca taking into account two distinct phases of its ontogenetic development (i.e. newborn and adult specimens) and the marked sexual dimorphism recently reported on its venom proteome. Proteomic data analysis showed wider dynamic range for toxins when comparing to non-toxins and a dynamic proteome rearrangement in cellular proteins upon B. jararaca development. Differentially expressed proteins covered a number of biological pathways related to protein synthesis, including proteins related to transcription and translation, which were found to be significantly higher expressed in the newborn venom gland. Our results showed that the variation in the expression levels of cellular proteins gives rise to an even higher variation in the dynamic range of the expressed toxins. Upon ageing, the molecular constraints related to protein synthesis together with ecological traits would likely have an impact on the toxin repertoire, which, in the case of B. jararaca species, would enable the species to deal with different prey types during its lifespan.

2016-09-28 | PXD004186 | Pride

The distinct N-terminomes of Bothrops jararaca newborn and adult venoms.

Project description:In this study we applied the Terminal Amine Isotopic Labeling of Substrates (TAILS) technology to characterize the natural N-terminome of newborn and adult Bothrops jararaca venoms.

2021-05-15 | PXD022696 | Pride

A Bothrops jararaca venom subproteome to benchmark a spectral clustering algorithm by controlling the selection bias

Project description:The clustering of mass spectra is a critical component of many proteomics applications. The clustering validation science is just as important, having evolved side by side with the clustering algorithms themselves. In this work, we build on Rieder et al. 's cluster validation framework, and we discuss the problem of selection bias in cluster validation measures; we introduce an assessment measure that is biased toward the number of peptide ion species; we introduce a cluster assessment framework for proteomics and demonstrate its importance by evaluating the performance of 8 clustering algorithms in 7 proteomics datasets, and we discuss the tradeoff between assessment measures. Finally, the validation methods presented here can be of broad applicability be-yond the clustering of mass spectra. This PRIDE entry describes in detail sample preparation, LC-MS/MS analysis, and protein identification of one of the proteomics datasets used in this work (> 10 kDa Bothrops jararaca snake venom proteome).

2022-03-16 | PXD022124 | Pride

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Project description:Venom gland transcriptome analysis of the Bothrops neuwiedi snake

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Characterization of extracellular vesicles from Bothrops jararaca snake venom

Project description:Venoms are important evolutionary keys and innovation for several animal taxons., including snakes. Their use is specially related to feed, predation and defense. Snake venoms are composed mainly by free secreted proteins, about 98%, and stored in the lumen of the venom gland, where they are processed through poorly understood mechanisms. In the perspective of protein-diversity, venoms undergo evolutionary pressure which generate a rapid evolutionary response, causing a great diversity in their toxin-components. We know now that extracellular vesicles exist in snake venom, although their biological role are still unknown. We believe that understanding EV-mediated effects could change our way of seeing envenoming, especially long-term sequelae. From what we know about EVs and snake venoms, there is a lot of potential of cross-organism communication occurrence between snakes and human victims. To advance in the comprehension of venom EVs function, we used fresh B. jararaca venom as our model. Fresh venom was fractionated by sequential centrifugation, resulting in two populations of vesicles (Bj-EVs). Purified Bj-EVs were analyzed by electron microscopy, NTA and proteomics. The interaction of Bj-EVs with mammalian cells was accessed by fluorescence and electron microscopy.

2023-03-10 | PXD037375 | Pride

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