Project description:Study of global gene expression differences in malignant melanoma cell lines with or without specific abberations in BRAF NRAS or CDKN2A. Expression .CEL files from Affymetrix HG-U133A 2.0 arrays using total RNA from 5 human cell lines derived from metastasized melanoma
Project description:Expression .CEL files from Affymetrix HG-U133A 2.0 arrays using DNA from 14 human cell lines derived from metastasized melanoma Study of global gene expression differences in malignant melanoma cell lines with or without specific abberations in BRAF NRAS or CDKN2A. UKRV-Mel-16a was wrongly described as Ma-Mel-16a in Blöthner et al. 2005
Project description:Four different concentrations (0, 0.75, 1.5, 3 pM) of genes were spiked in groups (group size 6-7 transcripts) Each concentration combination were spiked into a common backgroung sample (HeLa cell line) and hybridized to three HG-U133A Plus 2.0 Arrays. Keywords: Latin square design for HG-U133A Plus 2.0 GeneChips
Project description:In this study, we used the Affymetrix HG-U133A 2.0 GeneChip for deriving a multigenic classifier capable of predicting HCV+cirrhosis with vs without concomitant HCC. We studied gene expression in cirrhotic tissues with (N=16) and without (N=47) HCC. Keywords: cross-sectional
Project description:The objective of the experiment was to compare changes in the transcriptome induced by direct X-irradiation of cells, using the human K562 erythroleukemic, Me45 melanoma and HCT116 colon cancer cell lines. The transcript levels of K542 and Me45 RNA samples were measured using Affymetrix HG-U133A microarrays and for HCT116 with HGU 133A 2.0 in time points 1, 12, 24 h after irradiation and compared to the levels of RNA from control cells. Experiments were repeated twice for Me45 and K562 cells.
Project description:Allele call files from on 250K StyI SNP array using DNA from 60 human cell lines from metastasized melanoma and from 44 corresponding peripheral blood mononuclear cells (CEL and CHP files provided). Analysis of the 250K StyI arrays was performed for 60 malignant melanoma cell lines derived from metastatic tumors of melanoma patients with GCOS and GTYPE (Affymetrix). In addition, DNA from peripheral blood mononuclear cells (PBMC) was available for 44 of the 60 samples.