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The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense

ABSTRACT: The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense

PROVIDER: PRJNA264842 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1630040.fastq.gz	Fastqsanger.gz
	SRR1630050.fastq.gz	Fastqsanger.gz
	SRR1630060.fastq.gz	Fastqsanger.gz
	SRR1630070.fastq.gz	Fastqsanger.gz
	SRR1630180.fastq.gz	Fastqsanger.gz

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Similar Datasets

The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense [RNAi_mutants_smallRNA]

Project description:Here we describe the identification and regulation of a novel dsRNA virus in Colletotrichum higginsianum. High throughput sequencing of small RNAs and strand-specific RNA-seq was performed on single gene knock-out mutants created for each RNAi component gene: rdr1, rdr2, rdr3, dcl1, dcl2, ago1, and ago2, and the double mutant: ∆dcl1∆dcl2. De novo assembly of the ∆dcl1 RNA-seq data identified two contigs that represented the forward and reverse strands of an uncharacterized dsRNA virus, designated here as Colletotrichum higginsianum non-segmented dsRNA virus 1 (ChNRV1). We found increased presence of the viral RNA in the RNA-seq datasets of the ∆dcl1, ∆dcl1dcl2, and ∆ago1 strains, suggesting that these genes are required for control of the virus. We show that viral small RNAs co-immunoprecipitate with a 6xFLAG-3xHIS-tagged AGO1 protein by sequencing the small RNAs from immunoprecipitated fractions. Additionally, analyses of the small RNA datasets from the RNAi mutants revealed control of the virus through small RNA-mediated silencing required both AGO1 and DCL1.

2016-04-28 | GSE62705 | GEO

The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense [RNAi_mutants_RNAseq]

2016-04-28 | GSE62707 | GEO

The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense [AGO_IP_smallRNA]

2016-04-28 | GSE62706 | GEO

Colletotrichum higginsianum IMI 349063

Project description:The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense [AGO_IP_smallRNA]

| PRJNA264847 | ENA

Colletotrichum higginsianum IMI 349063

Project description:The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense [RNAi_mutants_RNAseq]

| PRJNA264846 | ENA

Colletotrichum higginsianum IMI 349063

Project description:The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense [RNAi_mutants_smallRNA]

| PRJNA264848 | ENA

Transcriptome of 4 developmental stages of Colletotrichum higginsianum during infection of Arabidopsis thaliana

Project description:Transcriptome of 4 developmental stages of Colletotrichum higginsianum during infection of Arabidopsis thaliana

2012-06-04 | GSE33683 | GEO

The RNAi machinery in Colletotrichum higginsianum has a role in antiviral defense

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-04-28 | GSE62708 | GEO

Colletotrichum higginsianum

Project description:Colletotrichum higginsianum Raw sequence reads

| PRJNA997767 | ENA

Transcriptome of 4 developmental stages of Colletotrichum higginsianum during infection of Arabidopsis thaliana

Project description:Transcriptome of 4 developmental stages of Colletotrichum higginsianum during infection of Arabidopsis thaliana 3 biological replicates per stage. The four stages are : in vitro apressoria (VA), penetrating apressoria (AP), Biotrophic hyphea from the biotrophic phase (BH) and the switch from biotrophic hyphea to necrotrophic phase(SW). Each biological replicate of the last three stages, the in planta stages, was two fold deeper due to plant contamination (Arabidopsis thaliana)

2012-06-04 | E-GEOD-33683 | biostudies-arrayexpress

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