Project description:Investigation of whole genome gene expression level changes of TE-1 transfected with PCDNA3.1-Pax2 ,compared to TE-1 transfected with PCDNA3.1.
Project description:Investigation of whole genome gene expression level changes of TE-1 transfected with PCDNA3.1-Pax2 ,compared to TE-1 transfected with PCDNA3.1. Compare PAX2 activated genes by analyzing mRNA profilings between pcDNA3.1 and pcDNA3.1-PAX2- transfected TE-1 cells.
Project description:Transcriptional profiling of breast cancer cell line MCF-7 stably transfected with pcDNA3.1-Gli1 comparing control ones transfected with CON
Project description:Evaluating the identity of ubiquitinated and ubiquitin-associated proteins tandem co-purifying non-covalently with wild-type (17 glutamine) human Huntingtin fragment, amino acids 1-502 C-terminally tagged with the HA peptide epitope, basally expressed from the CMV promoter within vector pcDNA3.1 transiently transfected into striatal precursor cell line St14A, significantly elevated above cells transfected with empty pcDNA3.1 vector negative control, in cells co-transfected with human FLAG-ubiquitin.
Project description:Transcriptional profiling of hPTTG1-/- HCT116 human colorectal cancer cells comparing hPTTG1-/- HCT116 cells transfected with pcDNA3.1, and with hPTTG1-/- HCT116 cells transfected with pcDNA3.1-hPTTG1 plasmid.
Project description:Genome wide identification of TBX1 sites of occupancy in thyroid cancer cell line BCPAP cells by comparing control BCPAP cells transfected with pcDNA3.1-Vector (cotains Myc tag) with cells transfected with pcDNA3.1-TBX1 (contains Myc tag). By obtaining over 30 Million bases of sequence from chromatin immunoprecipitated DNA, we generated genome-wide chromatin-state maps of TBX1 transfected. We find that transcription factor TBX1 exerts tumor suppressive properties through regulating multiple downstream targets involved in PI3K/Akt and MAPK/Erk signaling pathways.
Project description:Murine prostate cancer cells RM1 were transfected with a fakery murine MXRA7 cDNA in pcDNA3.1 vector. Controls were RM1 transfected with pcDNA3.1 vector. G418 resistant colonies were obtained, and gene expression profiling was performed using Agilent 4*44K array and dual labeling.
Project description:Transcriptional profiling of hPTTG1-/- HCT116 human colorectal cancer cells comparing hPTTG1-/- HCT116 cells transfected with pcDNA3.1, and with hPTTG1-/- HCT116 cells transfected with pcDNA3.1-hPTTG1 plasmid. Two-condition experiment, pchyg vs. 7-2 and 18-2 cells. 1 control, 2 different transfected cells.
Project description:MKN28 cell lines were transfected with pCDNA3.1-ZIC1 or pCDNA3.1 empty vector. Then, an Agilent oligonucleotide microarray was conducted to systematically determine downstream targets of ZIC1.
