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Mus musculus

ABSTRACT: Genome-wide RING1B binding in Fbxl10 overexpressing 3T3-L1 cells at day 2 of differentiation

PROVIDER: PRJNA270722 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1722820.fastq.gz	Fastqsanger.gz
	SRR1722814.fastq.gz	Fastqsanger.gz
	SRR1722815.fastq.gz	Fastqsanger.gz
	SRR1722816.fastq.gz	Fastqsanger.gz
	SRR1722817.fastq.gz	Fastqsanger.gz

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Genome-wide RING1B binding in Fbxl10 overexpressing 3T3-L1 cells at day 2 of differentiation

Project description:We analyzed RING1B binding regions in 3T3-L1 cell lines transduced with the retroviral vector for V5-tagged Fbxl10 or its dF-box mutant. RING1B ChIP-seq in empty, Fbxl10-1, and dF-box mutant vector transduced 3T3-L1 preadipocytes, in duplicate, and V5-Fbxl10 ChIP-seq in Fbxl10 overexpressing 3T3-L1 cells

2015-03-04 | E-GEOD-64312 | biostudies-arrayexpress

Expression data from Fbxl10 overexpressing 3T3-L1 cells

Project description:Target genes of Fbxl10 during 3T3-L1 adipogenesis was analyzed 3T3-L1 cells overexpressing Fbxl10 using retrovirus system containing LTR promoter were differentiated and RNA was extracted at day 2 of differentiation

2015-03-04 | E-GEOD-64154 | biostudies-arrayexpress

Genome-wide RING1B binding in Fbxl10 overexpressing 3T3-L1 cells at day 2 of differentiation

Project description:We analyzed RING1B binding regions in 3T3-L1 cell lines transduced with the retroviral vector for V5-tagged Fbxl10 or its dF-box mutant.

2015-03-04 | GSE64312 | GEO

Mus musculus

Project description:Expression data from Fbxl10 overexpressing 3T3-L1 cells

| PRJNA270327 | ENA

Fbxl10 regulates PRC1 recruitment to CpG islands and H2A ubiquitination

Project description:Polycomb repressive complex 1 (PRC1) catalyzes H2A monoubiquitination (uH2A) and regulates pluripotency in embryonic stem cells (ESCs). However the mechanisms controlling PRC1 recruitment and activity are largely unknown. Here we show that Fbxl10 interacts with Ring1B and Nspc1, forming a non-canonical PRC1. We demonstrate that Fbxl10-PRC1 is essential for H2A ubiquitination in mouse ESCs. Genome-wide analyses reveal that Fbxl10 preferentially binds to CpG islands and co-localizes with Ring1B on Polycomb target genes. Notably, Fbxl10 depletion causes modest dissociation of Ring1B but a major loss of uH2A on target genes. Furthermore rescue experiments for Fbxl10 reveal that its DNA binding capability and integration into PRC1 are required for proper H2A ubiquitination. ES cells lacking Fbxl10, like previously characterized Polycomb mutants, show a severely compromised capacity for successful differentiation. Our results shed light on a novel mechanism how CpG islands regulate chromatin function by affecting polycomb recruitment and activity.

2012-12-31 | GSE37930 | GEO

Fbxl10 regulates PRC1 recruitment to CpG islands and H2A ubiquitination

2012-12-31 | E-GEOD-37930 | biostudies-arrayexpress

Expression data from Fbxl10 overexpressing 3T3-L1 cells

Project description:Target genes of Fbxl10 during 3T3-L1 adipogenesis was analyzed

2015-03-04 | GSE64154 | GEO

Detection of microRNA expression during 3T3-L1 pre-adipocyte differentiation using a custom made microarray chip.

Project description:Differentiation of 3T3-L1 cells into adipocytes involves a highly orchestrated series of events including clonal expansion, growth arrest and terminal differentiation. The mechanisms coordinating these different steps are not yet fully understood. Here we investigated whether micro (mi)RNAs play a role in this process. Microarray analysis was performed to detect miRNA expression during 3T3-L1 preadipocyte differentiation. Several miRNAs, including let-7, were up-regulated during 3T3-L1 adipogenesis. Ectopic introduction of let-7 into 3T3-L1 cells inhibited clonal expansion as well as terminal differentiation. The mRNA encoding high mobility group AT-hook 2 (HMGA2), a transcription factor that regulates growth and proliferation in other contexts, was inversely correlated with let-7 levels during 3T3-L1 cell adipogenesis, and let-7 markedly reduced HMGA2 concentrations. Knockdown of HMGA2 inhibited 3T3-L1 differentiation. These results suggest that let-7 plays an important role in adipocyte differentiation and that it does so in part by targeting HMGA2, thereby regulating the transition from clonal expansion to terminal differentiation. 3T3-L1 cells were induced to differentiation into mature adipocytes using a canonical DMI cocktail. The time point at two days after confluency of 3T3-L1 was defined as day 0. Samples were collected at day 0, day 1, day 4, and day 7. The expression of microRNAs at day 1, day 4, and day 7 was compared to that of day 0.

2010-05-18 | E-GEOD-16229 | biostudies-arrayexpress

Identification of LncRNAs and mRNAs involved in adipogenesis

Project description:Genome wide lncRNA and mRNA expression profiling at day 0 and day 4 after induction of adipocyte differentiation in C3H10T1/2 and 3T3-L1 cells were analyzed by Arraystar Mouse LncRNA Microarray V3.0

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Genome-wide profiling of Prmt5 binding during 3T3-L1 adipogenesis

Project description:This study aims to determine the unique role of Prmt5 during adipogenesis by profiling Prmt5’s chromatin binding sites genome-wide at three time points during 3T3-L1 differentiation.

2023-09-08 | GSE148117 | GEO

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