Project description:To investigate the effects of DHA (omega-3) treatment on affecting brain dysfunction and metabolic disorders by profiling the transcripome in the hypothalamus of male Bgn_KO mice (with and without DHA) at 13 weeks old.
Project description:Gene Expressoin Profile in the Hypothalamus and Liver of Male Bgn_KO, FMOD_KO and WT Mice at 13 Weeks Old, With and Without DHA (omega-3) Treatment
Project description:To investigate the effects of gene Fmod on affecting brain dysfunction and metabolic disorders by profiling the transcripome in the hypothalamus and liver of male Fmod_KO mice at 13 weeks old.
Project description:To investigate the effects of gene Bgn on affecting brain dysfunction and metabolic disorders by profiling the transcripome in the hypothalamus of male Bgn_KO and wild-type (WT, litter mate) mice at 13 weeks old.
Project description:C57BL/6J male mice (11 weeks old) were randomly divided into two groups fed either the control (Con) diet (LabDiet 5010) or fiber deficiency (FD) diet for 15 weeks.
Project description:A control group of male C57BL/6J mice were fed a 60% fat diet (Research Diets D12492i) for 8 weeks, resulting in “ad libitum obesity” (ALO). Leptin receptor-deficient B6.BKS(D)-Leprdb/J (“db/db”) male mice were fed a low-fat chow diet (PicoLab Rodent Diet 20; Purina Mills Inc). Once the average body weights of the two groups were equivalent, all mice were fasted for 24 hours, after which they were sacrificed and perigonadal adipose tissue (PGAT) was dissected out. PGAT was cultured ex vivo for 6 hours, after which conditioned media was collected and submitted for proteomic profiling. At the time of sacrifice, ALO mice were 17 weeks old and db/db mice were 10 weeks old. “HFD” is also used to refer to the ALO condition while “DB” is used to refer to the “db/db” condition.
