Project description:Transcriptome of A. nidulans R21 and ∆gprH strains when grown on MM+1% glucose for 24 hours and transferred to MM with no carbon for 4 and 8 hours
Project description:Transcriptome of A. nidulans R21 and ?gprH strains when grown on MM+1% glucose for 24 hours and transferred to MM with no carbon for 4 and 8 hours Three conditions: MM with glucose for 24 hours and MM without glucose for 4 and 8 hours. Two strains R21 and ?gprH. Three biological repetitions of each point.
Project description:Transcriptome of A. nidulans ∆pkaA strain when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours
Project description:Transcriptome of A. nidulans TNO2a3 and ∆ptpB strains when grown on minimal media plus casaminoacids and transferred to minimal media plus glucose as a sole carbon source for 4 hours
Project description:Transcriptome of A. nidulans TNO2a3, ∆snfA and ∆schA strains when grown on complete media (CM) and transferred to minimal media plus avicel as a sole carbon source for 8 and 24 hours.
Project description:Transcriptome of A. nidulans TNO2A3, ∆msbA and ∆MHD strains when grown on complete media (YUU) and transferred to minimal media plus avicel as a sole carbon source for 24 hours
Project description:In Aspergillus nidulans, nitrogen and carbon metabolism are under the control of wide-domain regulatory systems, including nitrogen metabolite repression, carbon catabolite repression. Transcriptomic analysis of the wild type strain grown under different combinations of carbon and nitrogen regimes was performed, to identify differentially regulated genes. Carbon metabolism predominates as the most important regulatory signal but for many genes, both carbon and nitrogen metabolisms coordinate regulation.
Project description:Glutathione (GSH) is an abundant and widely distributed antioxidant in fungi. Hence, understanding cellular GSH metabolism is of vital importance to deciphering redox regulation in these microorganisms. In this study, we generated dugB (AN1879), dugC (AN1092), and dugB dugC double deletion mutants which display disruption of the GSH degradation pathway in Aspergillus nidulans. Deletion of dugB, dugC or both resulted in a moderate increase in GSH content under growing conditions and substantially slowed down the depletion of GSH pools under carbon starvation. Inactivation of dug genes caused reduced accumulation of reactive oxygen species, decreased autolytic cell wall degradation and extracellular enzyme production, increased sterigmatocystin formation but decreased viability in starving cultures. Changes in the transcriptomes suggested that enzyme secretions were controlled at post transcriptional level. In contrast, secondary metabolite production was also regulated at the level of mRNA abundance. Based on these findings, we suggest that GSH connects starvation and redox regulation to each other: A. nidulans cells utilize GSH as stored carbon source during starvation. The reduction of GSH contents of cells alters the redox state activating regulatory pathways responsible for carbon starvation stress responses. Under glucose rich conditions, inactivation of dug genes reduced conidia production of surface cultures, disturbed sexual development and down-regulated the transcription of genes encoding MAP kinase pathway proteins (e.g. steC, sskB, pbsA, hogA, mkkA) or proteins involved in the regulation of conidiogenesis or sexual differentiation (e.g. flbA,C,E, nosA, rosA, nsdC,D). These finding indicates that the authority of redox regulation goes far beyond the protection against redox stress; it affects development, stress responses (other than redox stress) and secondary metabolism as well.
