Project description:Arabidopsis mutants in the PMP/PNP oxidase PDX3 show abberant growth and development.RNA sequencing reveals strong induction of stress-related genes in pdx3, particularly those associated with biotic stress.
Project description:Identification of differentially expressed genes in Arabidopsis thaliana mutants in response to combined abiotic stress treatment through Microarray experiment.
Project description:Ozone at an elevated level is an important environmental stress factor that limits plant growth and development. To test how O3-induced ROS signalling interacts with the ABA pathway we present a global characterization of O3-responsive genes in the abi1td mutant. To understand better ABA signalling and the interactions between stress-response pathways we also performed a microarray analysis of drought-treated abi1td and WT plants. Since ABA signalling is well known to mediate defined responses based on the WT and different mutants analysis in drought stress conditions, the comparison of the O3 and drought stress response in abi1td enabled the identification of new processes depending on ABA-related pathways in O3-treated plants. Altogether, our findings indicate that ABI1 plays the role of a general signal transducer linking diferrent hormone signalling pathways to O3 stress tolerance.<br><br><br><br>Key words: ROS signalling; ABA signalling; ozone stress; drought stress; environmental stress; gene knockout;
Project description:Common and distinct transcriptomic responses to moderate light and drought stress in the different mutants. We used microarrays to analyse the response untreated and moderate light and drought stress in aox1a mutants, rpoTmp mutants and aox1a:rpoTmp double mutants. This was analysed in paralell with WT (Col0) plants in order to identify the responses to stress.