Project description:To identify SET1A genome-wide occupancy and further unveil its role in transcriptional regulation in mouse ES cells, we carried out chromatin immunoprecipitation followed by high sequencing (ChIP-seq).We established a stable ES cell line expressing 2X Flag tagged SET1A and performed ChIP with anti-Flag M2 beads, followed by deep sequencing. We found that the SET1A peaks show an outstanding enrichment in promoter region. Importantly, these SET1A binding loci revealed a clear co-localization with OCT4, and high H3K4me3 level, which is consistent with its interaction with OCT4 and intrinsic H3K4 methylase activity.
Project description:Gene expression profiling reveals that SET1A regulates gene expression, metabolism and development programs in mouse ES Cells To analyze the global effect of SET1A on gene expression in ES cells, we compared the gene expression profiles in CGR8 cells treated with control and Set1A siRNA by RNA-seq.