Project description:Experiments to test the effect of CtBP2 inhibition on metabolism of breast cell lines. In particular, experiment 1 involves comparison between a normal breast cell line (MCF102A) and a triple-negative breast cancer cell line (MDA-MB231). Experiment 2 is a study between MDA-MB231 silenced for CtBP2 by stable RNA interference (shCtBP2 cells) compared to scramble (shCTRL cells). Experiment 3 is a comparison between a normal breast cell line (MCF102A) and a triple-negative breast cancer cell line (MDA-MB231)in the presence of the absence of small-molecule CtBP inhibitors: HIPP (400 μM) or P4 (300 μM)for 48 hours.
Project description:This experiment aims to globally identify LARP6 binding sites on the transcriptome by performing iCLIP in MDA-MB231 cell lines expressing GFP-LARP6. MDA-MB231 cell lines expressing GFP only were used as controls in the experiment.
Project description:Here, we performed N-glycoproteomics on six triple negative breast cancer cell lines (commercially available cell lines: HCC1187, HCC1937, MDA-MB157, MDA-MB231, MDA-MB436, MDA-MB468) and five normal control cell lines (commercially available MCF10A and 4 non-immortalized human mammary epithelial cells: HMEC_RM10, HMEC_RM1, HMEC_RM2, HMEC_HB5) using hydrazide-based enrichment. Quantitative proteomics and integrative data mining led to the discovery of Plexin B3 (PLXNB3) as a previously undescribed TNBC-enriched cell surface protein.
Project description:In this work of Kisaki et al, we analyze the variation of proteome responses upon treatment of breast cancer cell lines MCF7 and MDA-MB231 with Bothrops jararaca snake venom
Project description:RNA-Seq profiling of triple-negative MDA-MB-231 cell line with know-down of non-canonical WNT signaling receptor Ror1. The MDA-MB231 cells were either transfected with a non-sense control shRNA (shCTL) or with a ROR1 shRNA (shROR1) construct. The objective was to find expression-responsive targets of these perturbations as potential drivers of MDA-MB231 cell invasiveness.
Project description:RNA sequencing of MDA-MB231 and U2OS cancer cell lines exposed to the alkylating agent methyl methanesufonate (MMS) and classical chemotherapeutics
