Project description:ACN_BlumORF_20101027_F009873 In solution tryptic digest of ACN fractions and crude extracts of sporulating hyphae nRPLC 130 min 2-32%ACN and 20min 32-48%ACN gradients online with nESI LTQ OrbitrapXL MSMS (CID) db: Blumeria ORF db (contigs) Mascot search 99% confidence
Project description:Large scale proteomics of Blumeria graminis f.sp. hordei DH14 has been conducted with a new ORF database containing candidate secreted effector proteins (CSEPs). With a comparative approach, CSEPs only deteted in Haustoria (the interacting cells) containing tissue were identified.
Project description:Identification of avirulence genes in the barley powdery mildew fungus (Bgh) by RNA-sequencing and transcriptome-wide association analysis on a set of Bgh isolates
Project description:Conidiospores are the asexual propagation units of many plant-pathogenic fungi. In this article, we report an annotated proteome map of ungerminated conidiospores of the ascomycete barley powdery mildew pathogen, Blumeria graminis f.sp. hordei. Using a combination of two-dimensional polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry, we have identified the proteins in 180 spots, which probably represent at least 123 distinct fungal gene products. Most of the identified proteins have a predicted function in carbohydrate, lipid or protein metabolism, indicating that the spore is equipped for the catabolism of storage compounds as well as for protein biosynthesis and folding on germination.
Project description:We performed RNA-sequencing of Bgh-infected barley leaves at two different time-points after infection to examine gene expression in the barley powdery mildew isolate DH14 during plant pathogenesis.
