Project description:Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro THP-1 (ATCC TIB-202) cell line infection, comparing bacteria grown in host medium and intracellular bacteria recovered from infected THP-1 cells.
Project description:This SuperSeries is composed of the following subset Series: GSE32241: Differentially regulated genes induced in Mycobacterium avium subspecies paratuberculosis by in vitro acid-nitrosative multi-stress GSE32242: Differentially regulated genes induced in Mycobacterium avium subspecies paratuberculosis by in vitro infection of THP-1 human macrophage cell line Refer to individual Series
Project description:Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress.
Project description:Vaccination decreases bacterial shedding and reduces the incidence and severity of infection by Mycobacterium avium subsp. paratuberculosis (Map), the causative agent of paratuberculosis (PTB), a chronic granulomatous enteritis that economically impacts the dairy industry. An inactivated Map vaccine exhibits features suggestive of trained immunity in cattle, along with enhanced neutrophil (PMN) activation in rabbits. Although PMNs from healthy cattle exhibit antimicrobial effects against Map, the impact of vaccination on bovine PMN function remains undetermined. This study has further explored the mechanisms of trained immunity mediated by PMNs elicited by this vaccine in calves. PMN mediated phagocytosis, ROS production and NET release showed a significant increase in vaccinated calves upon stimulation in ex vivo assays. Furthermore, PMNs from vaccinated animals exhibited decreased motility that was partially restored upon ex vivo stimulation with Map. Upregulation of phagocytosis receptors such as TREM2 and PBDL1, genes related to NETosis TREM2, LYZ and F13A1 and downregulation of genes involved in chemotaxis, adhesion, and motility in vaccinated animals support these findings. Altogether, these findings support that this inactivated Map vaccine induces changes consistent with trained immunity in calves.
Project description:Bovine paratuberculosis is a serious chronic disease of the gastrointestinal tract caused by Mycobacterium avium subspecies paratuberculosis (MAP). The aim of this study was to detect proteomic changes in peripheral blood mononuclear cells (PBMC) from cows of the same herd with different MAP infection status after co-incubation with viable MAP in vitro using label-free LC-MS/MS. Our data contribute to a better understanding of the bovine immune response and mechanisms of susceptibility to MAP.
Project description:Comparing the transcriptomic responses between the Mycobacterium avium subspecies paratuberculosis (MAP) leuD mutant with the parent strain K-10 under different environmental stresses: nutrition, temperature, anaerobic conditions, high- and low- pH conditions.
Project description:The alteration in genomic expression profiles of mRNA and long non-coding RNA (lncRNA) in bovine macrophages in response to Mycobacterium avium subspecies paratuberculosis (MAP) infection was investigated by applying an RNA-Seq based approach. A computational pipeline was implemented which identified more than 300 unannotated lncRNAs in macrophages of which 38 were differentially regulated by the infection.
Project description:Comparative expression profiles of 3 defined groups of Mycobacterium avium subspecies paratuberculosis (MAP) infected terminal ileum from sheep. Defined by histopathlogy and IS900 real-time PCR as multibacillary, paucibacillary and asymptomatic sheep. Six sheep per group provided biological and technical replicates on the RIGUA custom array (Watkins et al., 2008).
Project description:Mycobacterium bovis (M. bovis) and Mycobacterium avium subspecies paratuberculosis (MAP) are important pathogens of cattle, causing bovine tuberculosis and Johne’s disease respectively. M. bovis and MAP infect residential macrophages in the lung and intestines respectively and subvert the macrophage biology to create a survival niche. To investigate this interaction we simultaneously studied the transcriptional response of bovine monocyte-derived macrophages to infection with two strains of M. bovis (AF2122/97 and G18) and two strains of MAP (C & L1).