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Aedes aegypti

ABSTRACT: Aedes aegypti salivary gland transcriptome from male and female adult organisms

PROVIDER: PRJNA298896 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR2659965_1.fastq.gz	Fastqsanger.gz
	SRR2659965_2.fastq.gz	Fastqsanger.gz
	SRR2659966_1.fastq.gz	Fastqsanger.gz
	SRR2659966_2.fastq.gz	Fastqsanger.gz

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Project description:Aedes aegypti is a major vector for dengue, chikungunya and yellow fever. Though salivary gland plays a crucial role in transmission of virus and vector life cycle, there is no global and unbiased published report on salivary gland proteome of Ae. aegypti. In this study, we have carried out mass spectrometry based proteomic analysis of salivary gland from adult female Ae. aegypti mosquitoes. By fractionating the proteins isolated from salivary gland on SDS-PAGE and analyzing the in-gel digested bands on high resolution mass spectrometry, we identified 1,205 proteins. This is by far the largest catalogue of salivary gland proteome of Ae. aegypti. These proteins were then assigned molecular functions and biological processes. Several immunity related pathways were found to be enriched in salivary gland. In addition, subset of proteins was predicted to secretory in nature and may play an important role during blood feeding. This study provides a useful resource of proteins expressed in salivary gland of Ae. aegypti female mosquitoes and will aid in biomedical research focused on development of transmission blocking vaccine.

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