Project description:Compared analysis of the transcriptomes of 12-day old seedlings from wild type Col-0 treated with NO for 15, 30 and 60 min vs untreated control seedlings. Samples were harvested 12 h after dawn of day 12 after sowing and seedlings were grown under long days (16 h light / 8 h darkness) photoperiodic conditions.
Project description:In this study we developed an experimental set-up to study variation in twilight length using recently developed 7-band LED lights with fully programmable spectra and sigmoidal ramping of light intensity in controlled growth chambers. We employed this set-up to gain a comprehensive understanding of the impact of changing twilight lengths on Arabidopsis thaliana (Col-0) plants at both physiological and molecular levels. We studied the effect of a wide range of twilight lengths (0 min, 15 min, 30 min, 60 min, 90 min) on plant growth.
Project description:Transcriptional profiling of Arabidopsis far-red light pulse treated seeds comparing luh mutant with wild type (Col-0). Seeds were imbibed within 1 hr under white light and treated far-red light pulse for 5 min followed by 12 hr dark incubation. Goal was to determine the effects of LUH as transcriptional co-regulator during seed germination process.
Project description:Wild type Candida albicans (CAI8 containing CIp10) cultures were grown in triplicate at 1 x 107 cells ml-1 in 50 ml SC-pH3.0 broth. Cultures were then treated with 0, 20, 120 or 300<br>mM acetic acid. Cells were harvested after zero, 10, 30, 60, 120, 210 and 300 min treatment. RNA isolated from triplicate biological replicates were compared with a standard pooled control (untreated cells).
Project description:Arabidopsis cell culture was treated with 300 µM iron-citrate. Samples were collected before (0), and 5, 15, 30 and 60 minutes after iron addition. A repetition was performed for 2 points of the time course: before and 30 min after iron addition. Samples are designed as follow: Exp 1: 0(1), 5, 15, 30(1) et 60. Exp 2: 0(2) et 30(2)
Project description:Measurement of expression levels as a time course after shifting temperature-sensitive splicing factor mutant cells from 23C to 37C. Analysis of WT SS330, prp17 null, prp17-1 and prp22-1 cells. Samples were analyzed at 0, 5, 15, 30, 60 and 120 min. Keywords = pre-mRNA splicing Keywords = time course Keywords = intron Keywords: time-course
Project description:Transcripitonal profiling of Escherichia coli K-12 BW25113 comparing cells with isooctane treatment at time point of 0, 10, 30 and 60 min with two biological replicates
