Project description:Trichuris muris is very closely related to the human parasite T. trichiura sharing cross reactive antigens. Moreover, it is a remarkably tractable model system for dissecting immune responses and host parasite relationships and is actively being investigated in a number of laboratories worldwide. T. muris is a naturally occurring nematode parasite of mice which resides in the caecum and colon and has a direct oral faecal life cycle. High-throughput sequencing of Trichuris muris transcriptome for de novo assembly of transcripts. The main objective of this project is to recognize genes expressed in given life stages. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/
Project description:We analysed the exosomes secreted by the nematode Trichuris muris. Two replicates of exosomes were analysed using a 5600+ mass spectrometer
Project description:We measured the gene expression differences in germinal centre B cells from day 13 Trichuris muris-infected mice in which MLL1 is conditionally deleted in mature B cells
Project description:We analysed the excretory/secretory (ES) products and the exosomes secreted by the nematode Trichuris muris. Two batches of ES and Optiprep fractions of exosomes were analysed using a 5600+ mass spectrometer
Project description:The intestine is a site of diverse functions including digestion, nutrient absorption, immune surveillance, and microbial symbiosis. As such, intestinal homeostasis is vital for overall wellbeing. Faecal microRNAs (miRNAs) offer valuable non-invasive insights into the transcriptional state of the intestine. However, typical faecal miRNA yields and profiles remain incompletely characterised. Here, we develop an optimised protocol for faecal miRNA detection, and describe a reproducible murine faecal miRNA profile across several studies by performing a meta-analysis. By examining faecal miRNA changes during chronic infection with the gastrointestinal helminth, Trichuris muris, we identify the altered expression of miRNAs associated with fibrosis, barrier integrity and wound healing. Fibrosis was confirmed in vivo, suggesting a role for these miRNAs in regulating wound healing during chronic infection where the production of classical wound healing Th2 cytokines are low. Further implementations of this technique can identify novel hypotheses and therapeutic strategies in diverse disease contexts.
Project description:Trichuris muris, the mouse whipworm is used as a laboratory model of the human parasite T. trichuira - a gastrointestinal nematode. Three laboratory isolates of T. muris exist: the E, J and S isolates. Previous data has shown that the S isolate survives to chronicity in C57BL/6 mice unlike the E and J isolates which are expelled. The ability of the S isolate to persist is still unclear. Microarray analysis of gut samples from mice infected with either the E or S isolates of T. muris for 7 days was used to determine early changes in gene expression that may later determine the outcome of infection.
Project description:This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/ The human-infective whipworm Trichuris trichiura is estimated to infect up to a billion people and is responsible for considerable morbidity, especially in children of developing countries. The closely related species T. muris is a naturally occurring nematode parasite of mice that serves as a remarkably tractable model system for dissecting immune responses and host-parasite relationships. Such studies are of relevance beyond parasitology as helminths have arguably had a significant impact on the evolution of the mammalian immune system. Both Trichuris species reside in the caecum and colon of the host where they burrow their front end for feeding into the intestinal mucosa, thereby breaching the mucus barrier and allowing access of the microflora directly to the epithelium. The interplay of intestinal helminths, the bacterial microflora and the host immune system is currently a research focus in various laboratories (Bancroft et al 2012). This study will study the transcriptional responses of the intestinal mucosa (caecum) from infected and uninfected mice.
