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H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [H31_ChIP-Rx]

ABSTRACT: H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [H31_ChIP-Rx]

OTHER RELATED OMICS DATASETS IN: GSE75855

PROVIDER: PRJNA305536 | ENA |

REPOSITORIES: ENA

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H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation

Project description:H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation

| PRJNA285088 | ENA

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [H31_ChIP-Rx]

Project description:The goal of this study is to understand the genome-wide alterations in chromatin landscapy induced by a histone mutation (H3.3 K36M) derived from chondroblastomas

2016-05-13 | GSE75855 | GEO

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [ChIP-Rx]

Project description:The goal of this study is to understand the genome-wide alterations in chromatin landscape induced by a histone mutation (H3.3K36M) derived from chondroblastomas

2016-05-13 | GSE69289 | GEO

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-05-13 | E-GEOD-69291 | biostudies-arrayexpress

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [Ring1b_ChIP]

Project description:The goal of this study is to understand the genome-wide alterations in chromatin landscape induced by a histone mutation (H3.3K36M) derived from chondroblastomas Examination of genome-wide Ring1b distribution in cells expressing H3.3WT and H3.3K36M.

2016-05-13 | E-GEOD-69286 | biostudies-arrayexpress

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [Cbx2_ChIP]

Project description:The goal of this study is to understand the genome-wide alterations in chromatin landscape induced by a histone mutation (H3.3K36M) derived from chondroblastomas Examination of genome-wide Cbx2 distribution in cells expressing H3.3WT and H3.3K36M.

2016-05-13 | E-GEOD-75856 | biostudies-arrayexpress

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [RNA_Chondroblastoma_H3K36M_H3WT]

Project description:The goal of this study is to understand the genome-wide alterations in chromatin landscapy induced by a histone mutation (H3.3 K36M) derived from chondroblastomas Examination of H3K36M mutations to H3WT Chondroblastomas.

2016-05-13 | E-GEOD-75853 | biostudies-arrayexpress

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation [Ring1a-1b_RNASeq]

2016-05-13 | E-GEOD-75854 | biostudies-arrayexpress

H3K36 mutations promote sarcomagenesis through genome-wide remodeling of H3K36 and H3K27 methylation

Project description:This SuperSeries is composed of the SubSeries listed below.

2016-05-13 | GSE69291 | GEO

Histone H3.3 G34 mutations alter histone H3K36 and H3K27 methylation in cis

Project description:High-throughput sequencing of numerous patient samples has identified a myriad of frequent mutations of epigenetic regulators in human cancers, including recently discovered mutations in histone-encoding genes. Lysine-to-methionine mutations such as H3K27M and H3K36M share a common mechanism of inhibiting methylation pathways at the genome-wide level to promote tumorigenesis. However, the mechanism underlying the molecular and cellular changes due to H3G34 alterations is yet to be determined. H3G34 itself is not post-translationally modified; however, G34 lies in close proximity to K36, which undergoes methylation during transcriptional elongation. In Hela cells, H3.3G34L/W mutations have no effect on global levels of methylation on H3K36, H3K27, or other major methylation sites on endogenous histone H3, which include both H3.3 and the canonical H3.1/H3.2 proteins. However, long exposures of the blots revealed that methylation on the ectopic Flag-H3.3 proteins are affected by G34 mutations: with di- and trimethylation on H3K36 and H3K27ac reduced whereas H3K27me3 increased in the G34L and G34W mutated H3.3 compared to the WT H3.3. ChIP-seq results showed that mutations of H3.3G34 affect methylation on H3K36 and H3K27 in cis. G34L/W mutants abolish SETD2 methylation of H3K36. In contrast, the enzymatic activities of both EZH2 and p300 on H3K27 are not affected by G34 mutations. Consistent with changes in H3K27me3 and H3K36m3 levels, we found increased binding of PRC2 (EZH2, SUZ12 and EED) and PRC1 complex components (CBX8 and RING2) and reduced binding of H3.3K36me3 reader such as ZMYND11 to the G34 mutants. In summary, our study revealed that histone H3.3 G34 mutations alter histone K36 and K27 methylation in cis, and affect the binding of readers specific to K36 or K27 methylation.

2018-04-26 | GSE110389 | GEO

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