Project description:Analysis of change in transcriptosome after KLF2 and KLF8 knockdown in hADSC. Analysis of effects of siRNA transfection (siCtrl, siKLF2 or siKLF8) in hADSC at gene expression level.The hypothesis tested in the present study was that different KLF members have distinctive roles in these cells.
Project description:Analysis of change in transcriptosome after KLF4, KLF9, KLF11, KLF16 and KLF17 knockdown in hADSC. Analysis of effects of siRNA transfection (siCtrl, siKLF4, siKLF9, siKLF11, siKLF16 and siKLF17) in hADSC at gene expression level.The hypothesis tested in the present study was that different KLF members have distinctive roles in these cells.
Project description:The role of thyroid hormone receptors (TRs) in human adipose derived stem cells (hADSC), isolated from human adipose tissue collected during liposuction procedures
Project description:Human pluripotent cells were reset to ground state pluripotency by transient overexpression of NANOG and KLF2 and subsequent inhibition of ERK and protein kinase C. Transcriptional profiling of H9 parental and clonal lines, and induced pluripotent stem cells derived from adult fibroblast and adipose cell types was carried out in reset and conventional culture conditions.
Project description:In skeletal muscle differentiation, muscle-specific genes are regulated by two groups of transcription factors, the MyoD and MEF2 families, which work together to drive the differentiation process. Here we show that ERK5 regulates muscle cell fusion through Klf transcription factors. The inhibition of ERK5 activity suppresses muscle cell fusion with minimal effects on the expression of MyoD, MEF2, and their target genes. Promoter analysis coupled to microarray assay reveals that Klf-binding motifs are highly enriched in the promoter regions of ERK5-dependent upregulated genes. Remarkably, Klf2 and Klf4 expression are also upregulated during differentiation in an ERK5-dependent manner, and knockdown of Klf2 or Klf4 specifically suppresses muscle cell fusion. Moreover, we show that the Sp1 transcription factor links ERK5 to Klf2/4, and that nephronectin, a Klf transcriptional target, is involved in muscle cell fusion. Therefore, an ERK5/Sp1/Klf module plays a key role in the fusion process during skeletal muscle differentiation.
