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Global transcriptome changes after nitrogen replenishment following or not a rapamycin treatment in S288c yeast strain

ABSTRACT: Global transcriptome changes after nitrogen replenishment following or not a rapamycin treatment in S288c yeast strain

PROVIDER: PRJNA308574 | ENA |

REPOSITORIES: ENA

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Global transcriptome changes after nitrogen replenishment following a rapamycin treatment in S288c yeast strain

Project description:The study on the effect of nitrogen replenishment on the gene expression following a rapamycin treatment showed that the down-regulation of the glycolytic transcripts was generally suppressed after this treatment. This indicated that the TOR signaling pathway likely controls the expression of the glycolytic gene under these conditions.

2017-01-12 | GSE76716 | GEO

Global transcriptome changes after nitrogen replenishment in S288c yeast strain

Project description:The study on the effect of nitrogen replenishment on the gene expression showed that 390 genes were significantly upregulated (with a logFC ranging from 4.5 to 0.8), among which those related to RNA binding, ribosome binding, structural constituent of ribosome, transferase activity and transcription. 833 genes were significantly down-regulated (with a logFC ranging from -0.8 to -5.2) including those related to stress response, amino-acid catabolism process, protein catabolic process (ubiquitin-dependent and vacuolar protein degradation). But the most interesting and unexpected changes were those related to the down-regulation of the glycolytic transcripts. In fact, we observed a rapid down-regulation of many glycolytic mRNA (25/34) 30 min after nitrogen repletion. These data were surprising as expression of the glycolytic genes are generally considered as stable and sometimes they are even used as housekeeping control genes. In addition, the decoupling of the glycolytic gene expression from the expression of RP genes, ribosomal genes or those related to protein synthesis is unique to the present dataset as in most other data expression of these groups are tightly correlated when nutrients are available. This prompts us to examine in some details the factors which led to the control of the glycolytic mRNA instability in conditions of nitrogen replenishment.

2017-01-12 | GSE76715 | GEO

Global transcriptome changes after nitrogen replenishment following or not a rapamycin treatment in S288c yeast strain

Project description:This SuperSeries is composed of the SubSeries listed below.

2017-01-10 | GSE76778 | GEO

Saccharomyces cerevisiae S288C

Project description:Global transcriptome changes after nitrogen replenishment following a rapamycin treatment in S288c yeast strain

| PRJNA308443 | ENA

Saccharomyces cerevisiae S288C

Project description:Global transcriptome changes after nitrogen replenishment in S288c yeast strain

| PRJNA308444 | ENA

Comparing effects of rapamycin and caffeine on gene expression in YPD media

Project description:To identify genes influenced by rapamycin and caffeine in strains W303a, S288c, and Sigma 2000 Keywords: Drug dose response

2006-04-04 | GSE4584 | GEO

Dynamic mRNA gene expression following a rapamycin treatment

2015-04-07 | GSE54851 | GEO

RNA-seq of S. cerevisiae S288C wild-type and S288C-H2 mutant

Project description:S. cerevisiae strain S288C was subjected to CRISPR/Cas9 edition in the HMG-CoA reducatase gene using a modified housekeeping promoter ADH1 incorporating the up-stream region of TEF1 promoter (UADH1). Mutant strain was called S288C-H2.

2024-12-12 | E-MTAB-14650 | biostudies-arrayexpress

Dynamic mRNA gene expression following a rapamycin treatment

Project description:Dynamic mRNA gene expression from the wildtype YSBN6 during a rapamycin treatment (rapamycin-induced downshift). Rapamycin was added to yeast cells growing exponentially on glutamine as sole nitrogen source. A sample was taken at steady-state 10 minutes before , and then 3, 7, 10, 14, 24, 56 and 120 minutes after rapamycin treatment. Biological triplicate gene expression was measured for samples -10, 7 and 24 minutes after shift, for a total of 14 chips. Changes were generally evaluated relative to the steady-state point (-10 minutes). Biological variability can be assessed from the replicates time points. Other dynamic omics data are associated with this dataset. Consult the publication for more details.

2015-04-07 | E-GEOD-54851 | biostudies-arrayexpress

TORC1 signaling pathway inhibition by rapamycin and caffeine affect lifespan, global gene expression and cell proliferation of fission yeast

Project description:Target of rapamycin complex 1 (TORC1) is implicated in growth control and aging from yeast to humans. Fission yeast is emerging as a popular model organism to study TOR signaling, although rapamycin has been thought to not affect cell growth in this organism. Here we analyzed the effects of rapamycin and caffeine, singly and combined, on multiple cellular processes in fission yeast. The two drugs led to diverse and specific phenotypes that depended on TORC1 signaling pathway inhibition, including prolonged chronological lifespan, inhibition of global translation, inhibition of cell growth and division, and reprogramming of global gene expression mimicking nitrogen starvation. Rapamycin and caffeine differentially affected these various TORC1-dependent processes. Combined drug treatment augmented most phenotypes and effectively blocked cell growth. Although rapamycin showed a much more subtle effect on global translation than did caffeine, rapamycin was more effective in prolonging chronological lifespan. Rapamycin prolonged the lifespan of non-growing cells only when applied during the growth phase but not when applied after cells had stopped proliferation. The doses of rapamycin and caffeine strongly correlated with growth inhibition and with lifespan extension. This comprehensive analysis will inform future studies into TORC1 function and cellular aging in fission yeast and beyond.

2013-05-05 | E-MTAB-1489 | biostudies-arrayexpress

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