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Mus musculus

ABSTRACT: The histone demethylase KDM6A controls the mammary luminal lineage through enzyme-independent mechanisms

PROVIDER: PRJNA310874 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3145310.fastq.gz	Fastqsanger.gz
	SRR3145311.fastq.gz	Fastqsanger.gz
	SRR3145312.fastq.gz	Fastqsanger.gz
	SRR3145313.fastq.gz	Fastqsanger.gz
	SRR3145314.fastq.gz	Fastqsanger.gz

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Mus musculus

Project description:The histone demethylase KDM6A controls the mammary luminal lineage through enzyme-independent mechanisms

| PRJNA317447 | ENA

The histone demethylase KDM6A controls the mammary luminal lineage through enzyme-independent mechanisms

Project description:Mammary ducts and alveoli are composed of basal and luminal cells, with the fate and differentiation of secreting cells being controlled by hormones through specific transcription factors. This study establishes the essential role of the histone H3 lysine 27 trimethylation (H3K27me3) demethylase KDM6A (UTX) in a balanced basal and luminal cell compartment. Disproportionate formation of basal cells in the absence of KDM6A resulted in disorganized mammary ducts and alveoli and lactation failure. Mutant luminal progenitors lost their distinctive transcription factor expression pattern and acquired basal characteristics leading to a preferential expansion of this lineage. The structure of mammospheres obtained from mutant progenitors suggested they were derived from basal progenitors. The genomic H3K27me3 landscape was unaltered in the absence of KDM6A suggesting demethylase-independent mechanisms. In support of this, mammary tissue developed normally in mice expressing a catalytically inactive KDM6A. This study demonstrated that mammary luminal progenitor cells rely on UTX to stably maintain their identity and thereby establish a balance of basal and luminal cells required for a functional mammary gland.

2016-06-06 | GSE77586 | GEO

Mus musculus

Project description:KDM6A (UTX) controls the balance of basal and luminal mammary epithelium through regulating lineage-specific genes independent of its demethylase activity

| PRJNA306480 | ENA

Targeting KDM6A-KMT2D-p300 axis as a therapeutic strategy for diverse coronaviruses [RNAseq]

Project description:We demonstrate that the histone demethylase KDM6A promotes infection of diverse coronaviruses, including SARS-CoV-1, SARS-CoV-2, MERS-CoV and mouse hepatitis virus (MHV) in a demethylase activity-independent manner

2023-04-10 | GSE228268 | GEO

Alternative mRNA splicing controls functions of the human H3K27 demethylase UTX/KDM6A

Project description:The UTX/KDM6A gene encodes the UTX histone H3K27 demethylase, which plays an important role in mammalian development and is frequently mutated in cancers and particularly, in urothelial cancers. Using BioID technique, we explored the interactome of different UTX isoforms.

2024-01-28 | PXD041254 | Pride

Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [MSDK-Seq]

Project description:Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, and gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. Global profiling of differentially methylated regions in 2 cell types from 6 individuals.

2011-03-01 | E-GEOD-26139 | biostudies-arrayexpress

Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [SAGE-Seq]

Project description:Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, and gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. Global transcriptome profilings of 15 samples in normal breast tissue using Sage-Seq.

2011-03-01 | E-GEOD-26140 | biostudies-arrayexpress

Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [ChIP-Seq]

Project description:Here we describe the application of high-throughput sequencing technology for profiling histone and DNA methylation, and gene expression patterns of normal human mammary progenitor-enriched and luminal lineage-committed cells. We observed significant differences in histone H3 lysine 27 tri-methylation (H3K27me3) enrichment and DNA methylation of genes expressed in a cell type-specific manner, suggesting their regulation by epigenetic mechanisms and a dynamic interplay between the two processes that together define developmental potential. The technologies we developed and the epigenetically regulated genes we identified will accelerate the characterization of primary cell epigenomes and the dissection of human mammary epithelial lineage-commitment and luminal differentiation. Examination of histone H3K27me3 modifications in 2 cell types from 3 individuals and H3K4me3 modifications in 2 cell types from one individual sample.

2011-03-01 | E-GEOD-26137 | biostudies-arrayexpress

Genome-wide epigenome mapping in human induced pluripotent cells in WT and KDM6KO cells. [ChIP-Seq]

Project description:KDM6A is a histone demethylase that remove H3K27me3 lysine. This study compares the histone modification pattern between WT and KDM6A KO cells.

2022-03-03 | GSE168249 | GEO

Epigenetic regulation of cell type-specific expression patterns in the human mammary epithelium [SAGE-Seq]

2011-03-01 | GSE26140 | GEO