Project description:A study was designed to further define the viral landscape within Sjogren's Syndrome (pSS) affected salivary gland tissue to identify potential viral-mediated triggers in the pathogenesis of this autoimmune disease.
Project description:A study was designed to further define the viral landscape within Sjogren's Syndrome (pSS) affected salivary gland tissue to identify potential viral-mediated triggers in the pathogenesis of this autoimmune disease. Viral Probes for Vertebrate Infecting Viral Families
Project description:Sjogren's syndrome is an autoimmune disease, characterized by complaints such as xerostomia and keratoconjunctivitis sicca. In other autoimmune diseases such as diabetes and SLE, monocyte abberancies have been described. Therefore, this study aimed at studying the monocyte compartment in Sjogren's Syndrome, by transcription profiling of CD14+CD16- and CD14lowCD16+ monocytes in patients and controls.
Project description:Profiles of gene expression in salivary gland cells were compared between NOD/ShiLtJ mice, an animal model or Sjogren's syndrome with or without T1D. We used microarrays to detail the global programme of gene expression when type 1 diabetes and identified distinct classes of up- or down-regulated genes.
Project description:Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) and other respiratory viruses -Coronavirus OC43, Coronavirus 229E, Influenza A/H1N1, Influenza A/H3N2, Influenza B, Respiratory Syncytial Virus RSV A and RSV B - were analysed by bottom-up proteomics of viral cultures. High coverage of viral proteins was acheived after culturing in serum-free conditions when compared to cultures grown using standard conditions including 2% fetal bovine serum.
Project description:NOD.B10Sn-H2b/J mice are a mouse model of primary Sjogren's syndrome. We compared IgM and IgG autoantibody levels from NOD.B10Sn-H2b/J females with clinical disease to those of healthy controls.
Project description:This study aims at the quantitative analysis of human salivary extracellular vesicle (EV) content from patients affected by primary Sjogren's syndrome (pSS) in order to determine candidate biomarkers for disease diagnosis, patient stratification and monitoring.
Project description:We performed autoantigen arrays on sera derived from Sjogren's syndrome mice that lacked Myd88 systemically (NOD.B10LSL-/- strain) or lacked Myd88 in specific tissues (NOD.B10Myd88delta and NOD.B10LSL-/-Vav+ strains). We also examined sera from Myd88-sufficient controls (NOD.B10Myd88fl/fl, NOD.B10, and NOD.B10LSL+/- strains).
