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Danio rerio

ABSTRACT: Marker gene/pathway discovery for polystyrene particle toxicity in zebrafish larvae

PROVIDER: PRJNA311350 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3159070.fastq.gz	Fastqsanger.gz
	SRR3159080.fastq.gz	Fastqsanger.gz
	SRR3159071.fastq.gz	Fastqsanger.gz
	SRR3159081.fastq.gz	Fastqsanger.gz
	SRR3159072.fastq.gz	Fastqsanger.gz

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Marker gene/pathway discovery for polystyrene particle toxicity in zebrafish larvae

Project description:We use the zebrafish embryo model to study the innate immune response against polystyrene particles. Therefore, we injected 700nm polystyrene into the yolk at 2 dpf and took samples at 1 and 3 days post injection. This deep sequence study was designed to determine the gene expression profile by polystyrene particle toxicity. RNA was isolated from embryos at 1 and 3 days post injection. Wildtypes zebrafish embryos were micro-injected into the yolk (2dpf) with 1nl of 5mg/ml of 700nm red fluorescent polystyrene particles suspended in PVP (Polyvinylpyrrolidone) (n=3), mock injected with pvp (n=2), or Non-injected as a control (n=3). After injections embryos were transferred into fresh egg water and incubated at 28Â°C. At 1 and 3 days post injection 10 embryos per group were snap-frozen in liquid nitrogen, and total RNA was isolated using TRIZOL reagent.

2016-05-16 | E-GEOD-77755 | biostudies-arrayexpress

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