Project description:We screened the differential expressed microRNA from the rheumatoid arthritis patients and healthy person in their hydrops articuli CD4+ T cells. We screened the differential expressed microRNA from the rheumatoid arthritis patients and healthy person in their hydrops articuli CD4+ T cells. 1.Isolated the CD4+ T cells from the hydrops articuli cells of 2 rheumatoid arthritis patients. 2.Isolated the CD4+ T cells from healthy person's peripheral blood as control. 3.Isolation of total RNA from CD4+ T cells then sent the samples to KangChen Bio-tech Inc (Shanghai, China) where they were labeled, hybridized, scaned, normalied and transformed to the final data.
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
Project description:Leukocytapheresis((LCAP) is a safe, unique therapy prevalent for intractable rheumatoid arthritis even in case of drug allergy or infectious state. To investigate how to improve LCAP efficacy, we have conducted genes expressions analysis from peripheral blood of Rheumatoid arthritis patients using high resolution DNA microarray just before and after LCAP procedure. 8 Rheurmatoid arthritis patients have been compared just before and after Leukocytapheresis treatment (overall 16 samples). The Pre-LCAP samples were labeled using Cy5, and the Post-LCAP labeled with Cy3.
Project description:Leukocytapheresis((LCAP) is a safe, unique therapy prevalent for intractable rheumatoid arthritis even in case of drug allergy or infectious state. To investigate how to improve LCAP efficacy, we have conducted genes expressions analysis from peripheral blood of Rheumatoid arthritis patients using high resolution DNA microarray just before and after LCAP procedure.
Project description:Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic destructive arthritis. Although helper T cells are involved in the pathogenesis of RA, the characteristics of synovium-infiltrating CD4+ T cells are still largely unknown. In this study, we investigated synovium-infiltrating helper T cells of rheumatoid arthritis patients
