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Trypanosoma brucei

ABSTRACT: RNA-seq Transcriptome analysis of REG9.1 (Tb927.11.14220) when RNAi is induced, or not

PROVIDER: PRJNA322509 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3571760_1.fastq.gz	Fastqsanger.gz
	SRR3571760_2.fastq.gz	Fastqsanger.gz
	SRR3571761_1.fastq.gz	Fastqsanger.gz
	SRR3571761_2.fastq.gz	Fastqsanger.gz
	SRR3571762_1.fastq.gz	Fastqsanger.gz

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Trypanosoma brucei

Project description:RNA-seq Transcriptome analysis of REG9.1 (Tb927.11.14220) when overexpression is induced, or not using ectopic expression vector pLEW

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Trypanosoma brucei brucei

Project description:RNAi of TbNMD3 in procyclic form Trypanosoma brucei lines

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Project description:Three potential ELAV-like proteins of T. brucei, including Tb927.3.2930, Tb927.7.5380, and Tb927.8.6650, were either inhibited by RNAi or phenotypically activated by over-expression, followed by microarray analysis of the transcriptome. The results indicated that these ELAV-like proteins regulate the abundance of a large number of T. brucei transcripts, potentially through regulation of mRNA stability. Each of the three ELAV-like proteins were either inhibited by RNAi or over-expressed, in stable transgenic procyclic form cell lines. Total RNA was extracted 48h after tetracycline induction of the constructs (except for total RNA from Tb927.8.6650 RNAi which was extracted 24h after tet-induction), and sent to NimbleGen for cDNA synthesis and hybridization. Non-induced cells were analyzed in parallel.

2013-04-18 | E-GEOD-46161 | biostudies-arrayexpress

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Project description:Transcriptome-wide analysis of mRNA decay

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RNAi targeting ZC3H20 in differentiating EATRO1125 Trypanosoma brucei

Project description:RNAi was induced for 24h at the same tie as differentiation was induced.

2019-06-02 | E-MTAB-7995 | biostudies-arrayexpress

In vivo RNA interference RNAi target sequencing of Trypanosoma brucei

Project description:A direct comparison of RNAi in vitro with RNAi in vivo is being performed using RNA interference (RNAi) target sequencing (RIT-Seq) of Trypanosoma brucei to identify all genes specifically required for growth in vivo (the infectome). Assembly of the bloodstream-form T. brucei RNAi library and the RNAi target sequencing (RIT-seq) approach in African trypanosomes were reported previously in Alsford, S. et al. High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome. Genome Res 21, 915-924, 264 doi:gr.115089.110 [pii] 265 10.1101/gr.115089.110 (2011) and Alsford,S et al. High-throughput decoding of antitrypanosomal drug efficacy and resistance. Nature 482, 232236 doi:10.1038/nature10771 (2012). This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/

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Project description:RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650

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Project description:Gradient fractions of RNAi of XAC1 (Tb927.7.2780) in Trypanosoma brucei bloodstream forms. RNAi was induced using tetracycline and cell extracts were fractionated into polysomal and monosome-non-ribosome-associated fractions.

2017-12-16 | E-MTAB-6239 | biostudies-arrayexpress

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