Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Trypanosoma brucei

ABSTRACT: RNA-seq Transcriptome analysis of REG9.1 (Tb927.11.14220) when overexpression is induced, or not using ectopic expression vector pLEW

PROVIDER: PRJNA322511 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR3571753_1.fastq.gz	Fastqsanger.gz
	SRR3571753_2.fastq.gz	Fastqsanger.gz
	SRR3571754_1.fastq.gz	Fastqsanger.gz
	SRR3571754_2.fastq.gz	Fastqsanger.gz
	SRR3571755_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 8

Similar Datasets

Maps of histone variant H4.V deposition in Trypanosoma brucei brucei

Project description:Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where histone variant H4.V is deposited. A previously generated cell line (Siegel et al., 2009) in which both endogenous H4.V alleles are knockout out and ectopic overexpression of a Ty1-tagged version of H4.V can be induced was used. During the ChIP experiment, the DNA was digested with MNase to obtain mononucleosomes. Nucleosomes containing H4.V were pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and mononucleosomal DNA is purified and prepared for Illumina sequencing.

2018-08-24 | GSE118938 | GEO

Trypanosoma brucei

Project description:RNA-seq Transcriptome analysis of REG9.1 (Tb927.11.14220) when RNAi is induced, or not

| PRJNA322509 | ENA

Transcriptomics profiling of the developmental progression of Trypanosoma brucei

Project description:Mitochondrial metabolic remodeling is a hallmark of the Trypanosoma brucei digenetic life cycle since the insect stage utilizes the cost-effective oxidative phosphorylation to generate ATP, while bloodstream cells switch to less energetically efficient aerobic glycolysis. Due to difficulties in acquiring enough parasites from the tsetse fly vector for biochemical analysis, the dynamics of the parasite´s mitochondrial metabolic rewiring in the vector have remained obscure. Here, we took advantage of in vitro-induced differentiation to follow changes at the RNA levels.

2020-05-08 | GSE140796 | GEO

Trypanosoma brucei

Project description:Profiling of T. brucei rRNA Nm landscape using RibOxi-seq

| PRJNA397920 | ENA

Cell-based and multi-omics profiling reveal dynamic metabolic repurposing of mitochondria to drive developmental progression of Trypanosoma brucei

Project description:Mitochondrial metabolic remodeling is a hallmark of the Trypanosoma brucei digenetic life cycle since the insect stage utilizes the cost-effective oxidative phosphorylation to generate ATP, while bloodstream cells switch to less energetically efficient aerobic glycolysis. Due to difficulties in acquiring enough parasites from the tsetse fly vector for biochemical analysis, the dynamics of the parasite´s mitochondrial metabolic rewiring in the vector have remained obscure. Here, we took advantage of in vitro-induced differentiation to follow changes at the RNA, protein and metabolite levels. This multi-omics and cell-based profiling showed an immediate redirection of electron flow from the cytochrome mediated pathway to a mitochondrial alternative oxidase, an increase in proline consumption and its oxidation, elevated activity of complex II and certain TCA cycle enzymes, which led to mitochondrial inner membrane hyperpolarization and increased ROS levels in both mitochondrion and cytosol. Interestingly, these ROS molecules acted as signaling molecules driving developmental progression since exogenous expression of catalase, a ROS scavenger, halted the in vitro-induced cell differentiation. Our results provide insights into the mechanisms of the parasite´s mitochondrial rewiring and reinforce the emerging concept that mitochondria act as signaling organelles through release of ROS to drive cellular differentiation. Data availability:<a href='https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?&acc=GSE140796' rel='noopener noreferrer' target='_blank'>RNA-Seq</a>Proteomic data associated with this study are available in the PRIDE repository: accession number <a href='https://www.ebi.ac.uk/pride/archive/projects/PXD016370' rel='noopener noreferrer' target='_blank'>PXD016370</a>.

2020-08-20 | MTBLS1390 | MetaboLights

Trypanosoma brucei

Project description:RIP-Seq analysis of Trypanosoma brucei ELAV-like protein Tb927.8.6650

| PRJNA197395 | ENA

Trypanosoma brucei

Project description:Poly(A)-Specifc Ribonuclease 1 (PARN-1) Overexpression in Procyclic T. brucei

| PRJNA124829 | ENA

Trypanosoma brucei brucei

Project description:Trypanosoma brucei brucei VSG-seq

| PRJNA270648 | ENA

Trypanosoma brucei

Project description:Antisense Transcripts Impede Uridylation-induced Exonucleolytic Degradation to Generate Guide RNAs