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Mus musculus

ABSTRACT: Next Generation Sequencing of megakaryocyte-erythrocyte progenitor cells (MEPs) with or without mutations in JAK2 and Ezh2

PROVIDER: PRJNA323931 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3610490.fastq.gz	Fastqsanger.gz
	SRR3610500.fastq.gz	Fastqsanger.gz
	SRR3610481.fastq.gz	Fastqsanger.gz
	SRR3610491.fastq.gz	Fastqsanger.gz
	SRR3610501.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing of megakaryocyte-erythrocyte progenitor cells (MEPs) with or without mutations in JAK2 and Ezh2

Project description:Comparison of mRNA expression profiles of MEPs with or without mutations in JAK2 and Ezh2 by RNA sequencing. MEPs mRNA was extracted from six different transgenic mice (SclCre, SclCre;Ezh2+/-, SclCre;Ezh2-/-, SclCre; JAK2V617F, SclCre; JAK2V617F;Ezh2+/-, SclCre; JAK2V617F;Ezh2-/-) 10 weeks after tamoxifen injection. Our study represents the first detailed analysis of mRNA expression profile of MEP with or without mutations in JAK2 and Ezh2 , with biologic replicates, generated by RNA-seq technology. Our results revealed that mRNA expression profile of MEP with different genotype showed specific gene expression patterns, which allows to do biological comprehensive and quantitative analysis for hematopoiesis. MEPs mRNA profiles six different transgenic mice (SclCre, SclCre;Ezh2+/-, SclCre;Ezh2-/-, SclCre; JAK2V617F, SclCre; JAK2V617F;Ezh2+/-, SclCre; JAK2V617F;Ezh2-/-) were generated by deep sequencing.

2016-06-24 | E-GEOD-82072 | biostudies-arrayexpress

Next Generation Sequencing of megakaryocyte-erythrocyte progenitor cells (MEPs) with or without mutations in JAK2 and Ezh2

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Gene expression comparisons of megakaryocyte-erythrocyte progenitors isolated from wild type and c-MybTG/c-MybTG mice

Project description:Transgene insertion in proximity to the c-Myb gene disrupts c-Myb expression in megakaryocyte-erythrocyte progenitors (MEPs), resulting in skewed commitment to megakaryocyte lineage. To understand the genes regulated by c-Myb at the megakaryocyte-erythrocyte bifurcation, MEPs of c-MybTG/c-MybTG mice were examined. Detailed analysis on c-MybTG/c-MybTG is descried in the paper by Mukai et al, Mol Cell Biol 2006. The IL-7R-Lin-Sca-1–c-Kit+ fraction was subdivided into FcR loCD34+, FcRloCD34-, and FcRhiCD34+ populations. Among them, FcRloCD34- fraction was use as MEPs. MEPs from 16 wild type mice and those from 32 c-MybTG/c-MybTG mice were pooled and used for RNA purification.

2011-01-10 | E-GEOD-19901 | biostudies-arrayexpress

Gene expression comparisons of megakaryocyte-erythrocyte progenitors isolated from wild type and c-MybTG/c-MybTG mice

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Project description:Expression data from Ezh2-null erythrocyte/megakaryocyte progenitor (MEP)

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Hematopoietic Stem Cell Heterogeneity in JAK2 V617F+ Essential Thrombocythemia

Project description:In this study, we explored the transcriptome of hematopoietic stem cells (HSCs) and megakaryocyte-erythroid progenitors (MEPs) in JAK2 V617F+ PV and ET and found that distinctive gene expression patterns within MPN subtypes begin at the HSC stage. HSCs from ET exhibited prominent megakaryocyte (Mk)-lineage priming. The differentially expressed genes (DEGs) indicated that cellular processes and signaling pathways in HSCs and MEPs from healthy donors (HDs), PV and ET patients are differentially modulated

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Myeloproliferative neoplasms show different signature in megakaryocyte-erythroid progenitors among different subtypes

Project description:In this study, we explored the transcriptome of megakaryocyte-erythroid progenitors (MEPs) in JAK2 V617F+ PV and ET and found distinctive gene expression patterns within MPN subtypes. The differentially expressed genes (DEGs) indicated that cellular processes and signaling pathways in MEPs from healthy donors (HDs, or NCs) are differentially modulated in PV and ET patients.

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2016-06-24 | E-GEOD-82073 | biostudies-arrayexpress

Characterisation of the myeloid differentiation process of human hematopoietic stem cells

Project description:Hematopoietic stem cells (HSCs) can self-renew and/or differentiate into various functionally divergent progenitor cell types, such as common myeloid progenitors (CMPs), megakaryocyte-erythrocyte progenitors (MEPs) or granulocyte-macrophage progenitors (GMPs). When the process of self-renewal and differentiation is altered, e.g. upon genetic or epigenetic changes in HSCs, abnormal (pre)leukemic stem cell subpopulations may form, eventually resulting in the onset of hematological malignancies. To gain insight into the physiology and subsequent patho-physiology of self-renewal and differentiation, we performed RNA sequencing as well as proteomics analyses of human HSCs and downstream (myeloid) progenitor cells.

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