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Cut-and-Run in situ factor profiling maps DNA binding and 3D contact sites at high resolution

ABSTRACT: Cut-and-Run in situ factor profiling maps DNA binding and 3D contact sites at high resolution

PROVIDER: PRJNA329356 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3925780_1.fastq.gz	Fastqsanger.gz
	SRR3925780_2.fastq.gz	Fastqsanger.gz
	SRR3925790_1.fastq.gz	Fastqsanger.gz
	SRR3925790_2.fastq.gz	Fastqsanger.gz
	SRR3925800_1.fastq.gz	Fastqsanger.gz

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Cut-and-Run in situ factor profiling maps DNA binding and 3D contact sites at high resolution

Project description:Unlike Chromatin Immunoprecipitation (ChIP), which fragments and solubilizes total chromatin, Cut-and-Run is performed in situ, allowing for both high-resolution chromatin mapping and probing of the local chromatin environment. When applied to yeast and human nuclei, Cut-and-Run yielded precise transcription factor profiles while avoiding cross-linking and solubilization issues. Cut-and-Run is simple to perform and at low temperatures is inherently robust, with extremely low backgrounds that make it especially cost-effective for transcription factor and chromatin profiling. When used in conjunction with native ChIP-seq and applied to human CTCF, Cut-and-Run mapped high-resolution 3D directional interactions. We conclude that Cut- and-Run is a suitable complement or replacement for ChIP-seq that can also provide 3D mapping information.

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