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Bos taurus

ABSTRACT: Small RNA sequencing of large dense-core vesicle in bovine chromaffin cells

PROVIDER: PRJNA335347 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR3953057.fastq.gz	Fastqsanger.gz
	SRR3953058.fastq.gz	Fastqsanger.gz
	SRR3953059.fastq.gz	Fastqsanger.gz

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Small RNA sequencing of large dense-core vesicle in bovine chromaffin cells

Project description:Objectives: MicroRNA (miRNA) can be released to the extracellular medium and participates in neuronal communication. We investigate the mechanisms of miRNA exocytosis by vesicle fusion as a neuromodulator in a manner that are disparate from silencing gene expression. Methods: Small RNA sequencing data of large dense-core vesicle were generated by next-generation sequencing (NGS) in triplicate using Illumina Hiseq 2500. Results: Large dense-core vesicles contain a variety of known and novel miRNAs inside including miR-375. Conclusion: miRNAs can be novel neuromodulators, which are stored in LDCVs and released by vesicle fusion by SNARE assembly and synaptotagmin-1

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Transcription profiling of Bos taurus granulosa and theca cells during ovarian antral follicle development.

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