Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Mus musculus

ABSTRACT: Proteome and secretome analysis reveals differential post-transcriptional regulation of Toll-like receptor responses

OTHER RELATED OMICS DATASETS IN: PXD004113

PROVIDER: PRJNA338478 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Exploration and clinical validation of molecular typing of early colorectal cancer

Project description:Interventions: Case series:None Primary outcome(s): exon genes;transcriptional expression;proteome;protein phosphorylation group Study Design: Sequential

| 2739647 | ecrin-mdr-crc

Host-pathogen dynamics through secretome analysis of stimulated macrophages

Project description:The Toll-like receptors (TLRs) recognize different pathogen associated molecular patterns (PAMPs) and promote MyD88 dependent and independent responses. Previously, we have reported the discovery of the temporal changes in signaling cascades of macrophage proteome and secretome post-stimulation with different PAMPs. Here we present strategies to profile the secretome of TLR2-, TLR4, and TLR7- stimulated macrophages using whole pathogens were developed. Stable isotope labeling with amino acids in cell culture of macrophages was integrated with whole pathogen macrophage stimulation and subsequent targeted proteomics to quantify cytokines, chemokines, and transcription factors.

2022-02-28 | PXD008214 | Pride

miRNA-Seq analysis of the early phase of BoMac cell stimulation with a viral and a bacterial pathogen associated molecular patterns

Project description:Purpose: To examine differential expression of miRNA in bovine macrophages under the influence of Toll-like receptor agonists. Goal: to study and understand the miRNA regulation of early phase of transcriptomic changes induced by Toll-like receptor in bovine macrophage cell line.

2020-11-30 | GSE111162 | GEO

Proteome and Secretome Analysis Reveals Differential Post-Transcriptional Regulation of Toll-Like Receptor Responses

Project description:Toll-like receptors (TLRs) are essential sensors in innate immunity and among the first to detect invading pathogens. Many studies of TLR responses have focused on the intracellular signaling events that occur upon receptor stimulation. However, proteins released from the cell play a key role in cell-cell communication during an immune response. We have used mass spectrometry-based proteomic methods to investigate both the intracellular and extracellular responses of macrophages stimulated with individual TLR2, TLR4, and TLR7 ligands and whole pathogens. We performed global quantitative analyses of the mouse macrophage proteome and secretome using stable isotope labeling with amino acids and high-resolution mass spectrometry.

2017-03-06 | PXD004113 | Pride

Transcriptional Landscape of B cell Early Activation

Project description:The physiological responses to B cell receptors (BCR) and Toll-like receptors (TLRs) so vital to immunity are well known but the transcriptional signatures and regulatory mechanisms that initiate activation and release cells from quiescence remain unclear. Here, we show that BCR- or TLR-mediated activation of B cells involves a large shared transcriptional signature and a smaller subset of distinct signal-specific transcriptional responses. Signal-specific transcription is observable within 2 hours of ligand exposure; suggesting different modes of activation begin soon after ligand binding and long before the well-documented BCR and TLR-dependent physiological responses occur. Ligand-specific differences in regulatory mechanisms including RNA Pol II recruitment, activating (H3K4me3) and repressing (H3K27me3) histone marks, transcription factor binding sites in responsive gene promoters, and miRNA expression were observed. These results begin to define the transcriptional landscape of early B cell activation revealing more ligand-specific regulation and character than occurs much earlier than previously expected. CD43- mouse resting B cells were stimulated with ligands against the B cell receptor and TLR4 (LPS). RNA-sequencing was performed to describe differential transcription and ChIP-sequencing was performed to describe regulatory mechanism responses.

2015-04-30 | E-GEOD-61608 | biostudies-arrayexpress

Quantifying post-transcriptional regulation in the development of Drosophila melanogaster [s2r]

Project description:Proteome and transcriptome often show poor correlation, hindering the system-wide analysis of post-transcriptional regulation. Here, the authors study proteome and transcriptome dynamics during Drosophila embryogenesis and present basic mathematical models describing the temporal regulation of most protein-RNA pairs.

2018-10-13 | GSE121161 | GEO

Quantifying post-transcriptional regulation in the development of Drosophila melanogaster [devel]

2018-10-13 | GSE121160 | GEO

Ehrlichia chaffeensis str. Arkansas

Project description:Ehrlichia chaffeensis Transcriptome in Mammalian and Arthropod Hosts Reveals Differential Gene Expression and Post Transcriptional Regulation

| PRJNA140373 | ENA

Standardized whole-blood transcriptional profiling enables the deconvolution of complex induced immune responses

Project description:The study employs a standardized whole-blood stimulation systems to test the hypothesis that responses to Toll-like receptor ligands or whole microbes can be defined by the combined transcriptional signatures of key effector cytokines.

2016-08-04 | GSE85176 | GEO

Transcriptomic analysis of the early phase of BoMac cell stimulation with a viral and a bacterial pathogen associated molecular patterns.

Project description:Purpose: To examine differential expression of genes in bovine macrophages under the influence of Toll-like receptor agonists. Goal: to study and understand the early phase of transcriptomic changes induced by Toll-like receptor in bovine macrophage cell line.

2018-12-22 | GSE106843 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data