Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA352743 | ENA |
REPOSITORIES: ENA
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Project description:Two HPV(+) head and neck cancer cell lines (UPCI-SCC-090, UM-SCC-104), one HPV(–) head and neck cancer cell line (FaDu) and one nasopharyngeal epithelial cell line (NP69SV40T) were subjected to RNA-seq analysis.
2023-01-06 | GSE211322 | GEO
Project description:Microarrays were used to examine gene expression differences between human head and neck squamous cell carcinoma cell lines (FaDu, UTSCC8, UTSCC42a) grown in culture in comparison to a normal oral epithelial cell line. Gene expression data was integrated with global protein expression of head and neck squamous cell carcinoma cell lines and conditioned media to identify secreted protein markers up-regulated at the mRNA level in cancer cells versus the normal cell line. Total RNA obtained from head and neck squamous cell carcinoma cell lines and a normal oral epithelial cell line
2012-08-28 | E-GEOD-40185 | biostudies-arrayexpress
Project description:Microarrays were used to examine gene expression differences between human head and neck squamous cell carcinoma cell lines (FaDu, UTSCC8, UTSCC42a) grown in culture in comparison to a normal oral epithelial cell line. Gene expression data was integrated with global protein expression of head and neck squamous cell carcinoma cell lines and conditioned media to identify secreted protein markers up-regulated at the mRNA level in cancer cells versus the normal cell line.
2012-08-28 | GSE40185 | GEO
Project description:We demonstrate that GLUT4 up-regulation significantly increased cell migration and invasion in lower magligance head and neck cancer cell lines in vitro. We used microarrays to analyze the GLUT4 regulated gene expression underlying invasion-metastasis cascade.
2017-03-01 | GSE89631 | GEO
Project description:Transcriptome analysis in head and neck cancer cell lines, FaDu and UMSCC47 with and without 5-aza'2-deoxycytidine(Aza)/trichostatin A(TSA) treatment.
2018-05-10 | GSE98805 | GEO
Project description:The critical role of Bmi1 in promoting stem cell properties has been shown in different type of human cancers. Here, we established four stable clones to study Bmi-regulated miRNA expression patterns in head and neck caners. Bmi1-overexpressing cell lines (FaDu- Bmi1vs. FaDu-pcDNA3 cell line), and knock-down of Bmi1 cell lines (OECM1-sh-Bmi vs. OECM1-sh-Bmi1 cell lines) were established and used for analyzing miRNA expression patterms in Bmi-regulatory mechanism.
2013-02-11 | E-GEOD-31910 | biostudies-arrayexpress
Project description:By employing a global gene expression profiling, we performed analysis of the molecular pathways which are deregulated in head-and-neck cancer cell lines FaDu, Cal33 and UTSCC5 after siRNA mediated knockdown of Oct4 comparing to Scrambled.
2021-07-01 | GSE173161 | GEO
Project description:Elevated expression and activity of the epidermal growth factor receptor (EGFR)/protein kinase B (Akt) signaling pathway is associated with development, progression and treatment resistance of head and neck cancer (HNC). Several studies have demonstrated that microRNA-7 (miR-7) regulates EGFR expression and Akt activity in a range of cancer cell types via its specific interaction with the EGFR mRNA 3′ untranslated region (3′-UTR). In the present study, we found that miR-7 regulated EGFR expression and Akt activity in HNC cell lines, and that this was associated with reduced growth in vitro and in vivo of cells (HN5) that were sensitive to the EGFR tyrosine kinase inhibitor (TKI) erlotinib (Tarceva). miR-7 acted synergistically with erlotinib to inhibit growth of erlotinib-resistant FaDu cells, an effect associated with increased inhibition of Akt activity. Microarray analysis of HN5 and FaDu cell lines transfected with miR-7 identified a common set of downregulated miR-7 target genes, providing insight into the tumor suppressor function of miR-7. Furthermore, we identified several target miR-7 mRNAs with a putative role in the sensitization of FaDu cells to erlotinib. Together, these data support the coordinate regulation of Akt signaling by miR-7 in HNC cells and suggest the therapeutic potential of miR-7 alone or in combination with EGFR TKIs in this disease. Differential gene expression in head and neck cancer cell lines HN5 and FaDu under conditions of 24 h miR-NC (negative control) vs. miR-7 treatment.
2012-08-16 | E-GEOD-40130 | biostudies-arrayexpress
Project description:We performed gene expression profiling of 39 head and neck cancer cell lines and 1 hela cell line, and classified them based on previous classification of head and neck squamous cell tumors from patients We performed gene expression profiling of 39 head and neck cancer cell lines and 1 hela cell line.
2015-09-01 | E-GEOD-52088 | biostudies-arrayexpress
Project description:Background: Amphoterin-induced gene and open reading frame 2 (AMIGO2) has been reported to play a detrimental role in multiple cancer types. However, the precise role of AMIGO2 in hypopharyngeal squamous cell carcinoma (HPSCC) remains elusive. Methods: Bioinformatic analysis was employed to identify the differential expression and prognostic value of AMIGO2 in the head and neck cancer. Proliferation, apoptosis, and migration assays were then conducted after short hairpin RNA-mediated knockdown of AMIGO2 in FaDu cell line and in xenograft mouse model. Co-immunoprecipitation and mass spectrometry analysis were performed to determine the protein interaction between AMIGO2 and CAPN2. Rescue experiments with respect to the overexpression of CAPN2 were also performed. RNA sequencing after AMIGO2 knockdown with and without CAPN2 overexpression were conducted, followed by pathway enrichment analysis. Results: AMIGO2 expression was up-regulated in head and neck cancer and correlated with a poor prognosis. Functionally, HPSCC cell proliferation and migration were significantly inhibited in vivo and in vitro upon AMIGO2 knockdown. AMIGO2 binds to CAPN2 in cell context, and overexpression of CAPN2 could effectively reverse the inhibition from AMIGO2 knockdown. Bioinformatics analysis based on RNA sequencing indicated that the interaction between AMIGO2 and CAPN2 might be involved in multiple signaling pathways. Conclusions: Our work suggests that AMIGO2 promotes the proliferation and migration of HPSCC by interacting with CAPN2, implying that AMIGO2 can be therapeutically targeted to treat HPSCC.
2022-04-15 | GSE200657 | GEO