Project description:The presence of HBeAg has been linked to an increased risk of HBV-realted HCC. However, the biological role of HBeAg in modulating host gene expression is poorly understood. Therefore, we performed a cDNA microarray in response to presence of HBeAg in Huh-7 cells. We used human primeview gene expression microarray chips to assess the host cellular mRNA expression profile in response to HBeAg in Huh7 cells
Project description:mRNA expression profile modified by stable transfection of microRNA mir-517a (MIR517A) in a human hepatocellular carcinoma cell line Huh-7 Keywords: Hepatocellular carcinoma, Expression array, microRNA microRNA mir-517a (MIR517A) was transfected to Huh-7 cells using GFP-expressing lentiviral vector. Infected cells were selected using flow cytometry and subjected to mRNA expression microarray experiment. The profiles were compared to controls cells infected with only GFP protein.
Project description:HCC cell line, Huh-7 cells and HCC-LM3 cells, was transfected with METTL5 sgRNA to knockout METTL5 expression., and check the downstream mRNA changes.
