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Oryza sativa cultivar:Indica

ABSTRACT: Nitric Oxide mediated transcriptional Modulation in rice under arsenic stress

PROVIDER: PRJNA357668 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5116470_1.fastq.gz	Fastqsanger.gz
	SRR5116470_2.fastq.gz	Fastqsanger.gz
	SRR5116463_1.fastq.gz	Fastqsanger.gz
	SRR5116463_2.fastq.gz	Fastqsanger.gz
	SRR5116464_1.fastq.gz	Fastqsanger.gz

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Project description:Nitric Oxide mediated transcriptional Modulation in rice under arsenic stress

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Microarray based differential transcriptomics study to understand arsenic-thiourea interaction in rice.

Project description:In our earlier study, thiourea has been shown to improve the growth of rice and reduce the arsenic load from aerial parts of the seedlings. We have also shown the applicability of thiourea to delineate the redox regulatory mechansisms under arsenic stress in rice. To move further, present microarray was performed to reveal the involvement of overall gene and regulatory netwrok under arsenic with/without thiourea treatment in rice.

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Oleic acid-dependent modulation of Nitric Oxide Associated 1 protein levels regulate nitric oxide mediate signaling in plant defense.

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Oryza sativa Indica Group

Project description:Transcriptional profiling of indica rice cultivar FR13A under salt stress condition.

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Polysome bound mRNA Seq analysis of tolerant (APO) and susceptible (IR64) variety of Oryza sativa cv. Indica under 50% drought

Project description:Plants evolved several acquired tolerance traits for drought stress adaptation to maintain the cellular homeostasis. The combination of constitutive and acquired traits governs drought tolerance, which is crucial for maintaining crop productivity under drought. Drought affects protein synthesis, to uncover the translational landscape with response to drought stress in rice, polysome bound mRNA sequencing at anthesis stage in resistant APO and sensitive IR64 genotypes were performed. Our results demonstrate that drought tolerant genotype maintains higher transcripts bound to poly-ribosomes which facilitate higher protien synthesis which impacted on photosynthesis, spikelet fertility, seed filing and yield under drought stress. We identified many novel LncRNAs and relevant genes associated with translation which can play important role in manitaing grain protein content with drought tolerance.

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Genome-wide Expression Profiles of Drought and High-salinity Stresses-responsive Genes in Rice (Oryza Sativa L. Indica)

Project description:Three rice major tissues, namely flag leaf, shoot and panicle, were involved in this study. Each tissue had two kinds stress treatment, drought and high salinity, in 3 different time courses. For drought treated samples, an additional water recovery was applied. Each experiment had three replicates. Keywords: Comparison of gene expression in three tissues with stress treatment and without treatment To globally elucidate potential genes involved in drought and high-salinity stresses responses in rice, an oligomer microarray covering 37,132 genes including cDNA or EST supported and putative genes was applied to study the expression profiling of shoot, flag leaf, and panicle under drought or high-salinity treatment. Three rice major tissues, namely flag leaf, shoot and panicle, were involved in this study. Each tissue had two kinds stress treatment, drought and high salinity, in 3 different time courses. For drought treated samples, an additional water recovery was applied. Each experiment had three replicates.

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Comparative transcriptome analysis of transporters, phytohormone and lipid metabolism pathways in response to arsenic stress in rice (Oryza Sativa L.)

Project description:Arsenic (As) is a carcinogenic metalloid that is a contaminant widely polluting rice paddy soils around the world. In order to gain better insight into molecular mechanism of rice exposed to As(III) stress, we used next-generation sequencing technology to acquire global transcriptome alteration and miRNA regulation in rice upon As(III) treatments. Our results suggest time course and As(III)-dosing treatments were devised. Cluster analyses show that root and shoot samples were differentially grouped. For roots, sub-clusters were more distinct in the dosage course whereas for shoots they were most recognizable for the time course treatments. Other than the significantly regulated gene expression in the heavy metal-responsive sulfur and glutathione metabolism pathways, the expression of genes related to heavy metal transportation, jasmonate biosynthesis and signaling pathways, lipid metabolism and gene transcription were sharply regulated, indicating that rice allocates energy and resources from growth to stress response under As(III) stress. In addition to the detection of previously identified stress-related miRNAs, we further discovered 36 new As(III)-responsive miRNAs. These results expand our understanding of As(III) stress mechanism to the As(III)-responsive mRNA and miRNA transcriptomes, which provide a foundation for subsequent functional research.

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