Project description:Gene expression response of Populus cell cultures subjected to methyl jasmonate feeding was analyzed using the Affymetrix poplar genome microarrays. Experiment Overall Design: Populus tremuloides suspension cells were treated with methyl jasmonate (MJ) in DMSO or with DMSO alone (C). Cells were harvested after 48 hours. Two biological replicates were obtained for each condition.
Project description:The Poplar transcriptome was analyzed in mycorrhizal root tips in contact with Laccaria bicolor for 2 weeks. During mycorrhization the roots were treated with either 250M-BM-5m ACC, 10nM JA or 500M-BM-5M SA and compared to untreated mycorrhiza or control roots without contact to L. bicolor. In addition the poplar mutants 35S::PttACO1 and 35S::Atetr1 were used We performed 27 hybridizations (NimbleGen) with samples derived from Populus tremula x Populus alba clone 717-1B4 control roots, untreated mycorrhiza, SA-treated mycorrhiza, ACC-treated mycorrhiza and JA-treated mycorrhiza (3 biological replicates each) as well as Populus tremula x Populus tremuloides T89 control roots, mycorrhiza, 35S::PttACO1 mycorrhiza and 35S::Atetr1-1 mycorrhiza (3 biological replicates). All samples were labeled with Cy3.
Project description:The expression of stress-related genes induced by feeding of chestnut moth larvae (Conistra vaccinii L.) was studied with Vitreoscilla hemoglobin-expressing (VHb) and non-transgenic hybrid aspen lines (Populus tremula x P. tremuloides). Besides the herbivore-injured leaves (L1), cDNA microarray analyses were conducted using uninjured leaves of hybrid aspen lines positioned above (A) and below (B) the herbivory exposed leaves.
Project description:Gene expression response of Populus cell cultures subjected to methyl jasmonate feeding was analyzed using the Affymetrix poplar genome microarrays. Keywords: Stress response
Project description:To identify the genes encoding the defense proteins and gain a deeper insight into the Ptt nectary transcriptom, poplar DNA microarrays (Affymetrix) were hybridized with RNA of extrafloral nectaries and nectary-free leaf sections Many plant species grow extrafloral nectaries and produce nectar to attract carnivore arthropods as defenders against herbivores. We studied in Populus how insect feeding feeds back on nectary development and activity. Two nectary types evolved with Populus trichocarpa (Ptr) and P. tremula x P. tremuloides (Ptt) were studied from their ecology down to the genes and molecules.
Project description:Aim of the project: Genome wide gene expression profiles across the cambial zone are analyzed in 35um resolution from wild type hybrid aspen (Populus tremula x tremuloides) and two independent LMX5::AtIPT7 over expressor transgenic Populus tree lines.
Project description:To identify the genes encoding the defense proteins and gain a deeper insight into the Ptt nectary transcriptom, poplar DNA microarrays (Affymetrix) were hybridized with RNA of extrafloral nectaries and nectary-free leaf sections Many plant species grow extrafloral nectaries and produce nectar to attract carnivore arthropods as defenders against herbivores. We studied in Populus how insect feeding feeds back on nectary development and activity. Two nectary types evolved with Populus trichocarpa (Ptr) and P. tremula x P. tremuloides (Ptt) were studied from their ecology down to the genes and molecules. 3 samples of leaves and 3 of nectaries from P. tremula tremuloides field-culture
