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Mus musculus

ABSTRACT: Gene expression profiles in retinas from miRNA183/182/96 cluster knockout mice versus wild type controls by RNA-seq

PROVIDER: PRJNA378599 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5328920_1.fastq.gz	Fastqsanger.gz
	SRR5328920_2.fastq.gz	Fastqsanger.gz
	SRR5328915_1.fastq.gz	Fastqsanger.gz
	SRR5328915_2.fastq.gz	Fastqsanger.gz
	SRR5328916_1.fastq.gz	Fastqsanger.gz

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Gene expression profiles in retinas from miRNA183/182/96 cluster knockout mice versus wild type controls by RNA-seq

Project description:Retinas from various developmental stages at postnatal p5, p11, and p27 from miRNA183/182/96 cluster knockout mice versus wild type controls were used to prepare total RNA for RNA-sequencing.

2017-08-22 | GSE95852 | GEO

Gene expression profiles in the retina from RTBDN knockout mice versus wild type controls by RNA-seq

Project description:Retinas from RTBDN knockout mice versus wild type controls were used to prepare total RNA for RNA-sequencing.

2020-12-08 | GSE139980 | GEO

The microRNA cluster miR-183/96/182 contributes to long-term memory in a protein phosphatase 1 dependent manner

Project description:Memory formation is a complex cognitive function regulated by coordinated synaptic and nuclear processes in neurons. In mammals, it is controlled by multiple molecular activators and suppressors, including the key signaling regulator protein phosphatase 1 (PP1). Here, we show that memory control by PP1 involves the miR-183/96/182 cluster, which is selectively regulated during memory formation. Inhibiting nuclear PP1 in mice brain or training in object recognition task similarly increases miR-183/96/182 expression in the hippocampus. Mimicking this increase by overexpressing miR-183/96/182 enhances object memory, while suppressing endogenous level of the cluster reduces it. This effect involves the modulation of many plasticity-related genes, and we identified HDAC9 as one of the functional targets. Further, PP1 controls miR-183/96/182 in a transcription-independent manner influencing processing of their precursors. These findings provide novel evidence for the role of miRNAs in memory formation and suggest the implication of PP1 in miRNAs processing in the adult brain.

2016-09-20 | GSE83707 | GEO

The miR-183/96/182 Cluster Regulates Sensory Innervation, Resident Myeloid Cells and Functions of the Cornea Through Cell type-specific Target Genes

Project description:The miR-183/96/182 cluster (miR-183C) is specifically expressed in sensory neurons and immune cells and modulates corneal immune/inflammatory response to bacterial infection. To uncover the roles of miR-183C in corneal homeostasis through its regulation of sensory neurons of the trigeminal ganglia (TG) and innate myeloid cells, we created miR-183C conventional knockout (KO), and sensory nerve-specific (SNS-CKO) and myeloid cell-specific conditional knockout (MS-CKO) mice. We performed 3'RNA sequencing in the TG and corneas of these knockout mice and their wild type (WT)-control littermates. To study specific functions of miR-183C in corneal resident myeloid cells (CRMCs), we isolated Csf1r-EGFP+ CRMCs from conventional KO and MS-CKO and their WT control mice. By comparison of the gene expression profiles of the KO or CKO vs their corresponding WT mice, we identified a series of differentially expressed genes. Bioinformatic analyses uncovered tissue- and/or cell-type-specific target genes of miR-183C. Functional annotation of the target genes revealed functions of miR-183C in TG and CRMCs.

2023-12-20 | GSE250213 | GEO

GNPS - E. Coli Nissle wild type versus knockout strain

Project description:E. Coli Nissle wild type versus knockout strain grown in M-9 media.

2019-01-31 | MSV000083387 | GNPS

Modulation of the microRNA cluster miR-183-96-182 expression by the Epstein-Barr virus latent membrane protein 1

Project description:Epstein-Barr virus (EBV) is an oncogenic human herpesvirus involved in the pathogenesis of Burkitt’s lymphoma (BL) and various other lymphoproliferative disorders. In BL, EBV protein expression is restricted to the EBNA-1, but small non-coding RNAs such as the EBERs and micro (mi)RNAs can also be detected. miRNAs play major roles in crucial processes such as proliferation, differentiation and cell death. It has recently become clear that alteration in the expression profile of miRNAs contribute to the pathogenesis of a number of malignancies. During latent infection, EBV expresses 25 viral pre-miRNAs and modulates the expression of specific cellular miRNAs, such as miR-155 and miR-146, which potentially play a role in oncogenesis. Here, we established the small RNA expression profiles of three BL cell lines. Using large-scale sequencing coupled to northern blot and real-time RT-PCR analysis validation, we demonstrated the differential expression of some cellular and viral miRNAs. High-level expression of the miR-183-96-182 cluster and EBV miR-BART cluster was significantly associated with EBV type I latency. This expression was not affected by viral reactivation since TGF-ß1 stimulation did not significantly change the miRNA profiles. However, the expression of latent membrane protein (LMP)-1 triggered down-regulation of the expression of the miR-183-96-182 cluster. We further show that this effect involves the AKT signaling pathway.

2015-09-25 | GSE65752 | GEO

Gene expression profiles in the retina from RTBDN knockout mice versus wild type controls by RNA-seq

Project description:Gene expression profiles in the retina from RTBDN knockout mice versus wild type controls by RNA-seq

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Mus musculus

Project description:Gene expression profiles in lens from KLPH/lctl knockout mice versus wild type controls by RNA-seq

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sRNA sequencing of liver from lean rats and ZDF rats treated with vehicle or colesevelam

Project description:Colesevelam is a bile acid sequestrant approved to treat both hyperlipidemia and type 2 diabetes, but the mechanism for its glucose lowering effects is not fully understood. The aim of this study was to investigate the role of hepatic microRNA’s as regulators of metabolic disease and to investigate the link between the cholesterol and glucose lowering effects of colesevelam. To quantify the impact of colesevelam treatment in rodent models of diabetes, metabolic studies were performed in Zucker Diabetic Fatty (ZDF) rats and db/db mice. Colesevelam treatments significantly decreased plasma glucose levels and increased glycolysis in the absence of changes to insulin levels in ZDF rats and db/db mice. High-throughput sequencing and real-time PCR were used to quantify hepatic miRNA and mRNA changes, and the cholesterol-sensitive miR-96/182/183 cluster was found to be significantly increased in livers from ZDF rats treated with colesevelam compared to vehicle controls. In summary, these results support that colesevelam likely improves glycemic control through hepatic miR-96/182/183, a mechanism that directly links cholesterol and glucose metabolism.

2018-07-10 | GSE116826 | GEO