ABSTRACT: Transcription rates in S. cerevisiae wild type haploid and polyploid cells as well as in whi5 and cln3 mutant cells using Genomic Run On (GRO)
Project description:Determination of transcriptional rates and mRNA levels during osmotic stress in S. cerevisiae wild type and cbc1/sto1 mutant cells using Genomic Run On (GRO)
Project description:The polyploid S. cerevisiae karyotypes were analyzed by array-CGH to identify the deletion or duplication of gene or chromosome during the strain construction and after experimental evolution.
Project description:Transcriptional change by profiling yeast cells, comparing polyploid S. cerevisiae cells at 1000 generations with ancestral cells. Transcriptional change by profiling yeast cells, comparing both ancestral and evolved cells with diploid cells, stands for control.
Project description:We analyzed the effect of PUB1 and NGR1 on the transcription rates, mRNA stabilities and mRNA levels by doing GRO experiments in a deletion mutants comparing with a wild type in both glucose (YPD) and galactose (YPGal) media. Some data for the wt in YPGal are not included because were already in GEO database: GSE1002 accession number. This study focus on the transcriptional activity of RNA pol II in yeast genes. S. cerevisiae cells grown in YPD or YPGal to exponential phase were subjected to Genomic Run On. Data were normalized by the ArrayStat software. Home-made macroarrays containing 6035 ORF were used.
Project description:We analyzed the effect of the cell volume on transcription rate using the Genomic Run-on assay on polyploid and mutant cells of different cell volumes
Project description:We analyzed the effect of SAC3, SUS1 and SRC1 on the transcription rates, mRNA stabilities and mRNA levels by doing GRO experiments in a deletion mutants and the partial C-truncated version of Sac3 comparing with a wild type. Some data for mRNA amounts (sus1 ans src1 mutants) are not included because were already in GEO database: GSE920 and GSE6370 accession numbers. This study focus on the transcriptional activity of RNA pol II in yeast genes. S. cerevisiae cells grown in YPD to exponential phase were subjected to Genomic Run On. Data were normalized by the ArrayStat software. Home-made macroarrays containing entire ORFs were used.
