ABSTRACT: Transcription rates in S. cerevisiae wild type haploid and polyploid cells as well as in whi5 and cln3 mutant cells using Genomic Run On (GRO)
Project description:Determination of transcriptional rates and mRNA levels during osmotic stress in S. cerevisiae wild type and cbc1/sto1 mutant cells using Genomic Run On (GRO)
Project description:The polyploid S. cerevisiae karyotypes were analyzed by array-CGH to identify the deletion or duplication of gene or chromosome during the strain construction and after experimental evolution.
Project description:Genomic Run On (GRO): determination of the nascent transcriptional rates and mRNA levels in several yeast mutants (SAC3, SUS1, SRC1)
Project description:Transcriptional change by profiling yeast cells, comparing polyploid S. cerevisiae cells at 1000 generations with ancestral cells. Transcriptional change by profiling yeast cells, comparing both ancestral and evolved cells with diploid cells, stands for control.
