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Saccharomyces cerevisiae

ABSTRACT: CRAC of yeast RNA polymerase II in various thermosensitive strains at permissive and non-permissive temperature and anchor-away strains with the addition of rapamycin.

PROVIDER: PRJNA383311 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5454030.fastq.gz	Fastqsanger.gz
	SRR5454031.fastq.gz	Fastqsanger.gz
	SRR5454022.fastq.gz	Fastqsanger.gz
	SRR5454032.fastq.gz	Fastqsanger.gz
	SRR5454023.fastq.gz	Fastqsanger.gz

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CRAC of yeast RNA polymerase II in various thermosensitive strains at permissive and non-permissive temperature and anchor-away strains with the addition of rapamycin.

Project description:We obtained RNA polymerase II occupancy profile across the genome of S.cerevisiae in several strains, Wild type at 25 and 37 degrees C, RNA15-1 at 25 and 37 degrees C, anchor-away parental strain plus rapamycin, Reb1 anchor-away plus rapamycin, Rap1 anchor-away plus rapamycin, Nrd1-AID plus auxin, and nrd1-AID without auxin. This allowed us to compare polymerase profiles when these proteins are depleted from the nucleus.

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