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Transcription profiling of thermosensitive exosome mutants from permissive temperature and various time exposure to restrictive temperature

ABSTRACT: Transcriptome analyses of thermosensitive exosome mutants from permissive temperature to various time exposure to restrictive temperature.

INSTRUMENT(S): GenePix 4000A [Axon Instruments]

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: David Tollervey

PROVIDER: E-MEXP-537 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Microarray detection of novel nuclear RNA substrates for the exosome.

Houalla Rym R Devaux Frédéric F Fatica Alessandro A Kufel Joanna J Barrass David D Torchet Claire C Tollervey David D

Yeast (Chichester, England) 20060401 6

Microarray analyses were performed on yeast strains mutant for the nuclear-specific exosome components Rrp6p and Rrp47p/Lrp1p or the core component Rrp41p/Ski6p, at permissive temperature and following transfer to 37 degrees C. 339 mRNAs showed clearly altered expression levels, with an unexpectedly high degree of heterogeneity in the different exosome mutants. In contrast, no clear alterations were seen in strains lacking the cytoplasmic exosome component Ski7p. 27 mRNAs that were overexpressed ...[more]

PMID: 16652390

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