MRNA/miRNA expression profiles of human primary adipocytes exposed to low doses of bisphenols A, F and S
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PROVIDER: PRJNA385872 | ENA |
REPOSITORIES: ENA
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Project description:Bisphenols F (BPF) and S (BPS) are BPA structural analogs used in many marketed products as a replacement for BPA. Since sparse toxicological data are available yet for these bisphenol substitutes, our objective was to comprehensively characterize bisphenols gene targets in a human primary adipocyte model, using chronic exposure at two concentrations: a “low-dose” similar to the dose usually encountered in human biological fluids and a higher dose.
2017-05-09 | GSE98681 | GEO
Project description:This SuperSeries is composed of the SubSeries listed below.
2017-05-09 | GSE98682 | GEO
Project description:Bisphenols F (BPF) and S (BPS) are BPA structural analogs used in many marketed products as a replacement for BPA. Since sparse toxicological data are available yet for these bisphenol substitutes, our objective was to comprehensively characterize bisphenols gene targets in a human primary adipocyte model, using chronic exposure at two concentrations: a “low-dose” similar to the dose usually encountered in human biological fluids and a higher dose.
2017-05-09 | GSE98680 | GEO
Project description:The aim of the present study is to analyze by microarray techniques how gene expression is modulated after exposure to low and very low doses of ionizing radiation, to evaluate if the pattern of gene expression shows dose dependence, and to search for putative regulatory mechanisms behind the observed gene-expression modifications.For this, whole blood samples from five healthy donors diluted in IMDM (1:10) were exposed at six doses between 5 and 500 mGy. Total RNA extraction from CD4+ lymphocytes was done at four different post-irradiation times (150, 300, 450 and 600 min). After mRNA amplification, aRNAs were hybridized on 4x44k DNA microarrays. The results indicated that up-regulation were twice than down-regulation. Surprisingly, the number of modulated genes doesn’t seem to change drastically with dose, even at the lowest dose of 5 mGy. Clustering analysis revealed seven gene expression clusters with different dose dependence profiles. The functional analysis showed that the genes which increased their expression with the dose were related to p53 pathway and DNA damage response. This could be observed from 25 mGy, but becomes very clear at doses equal or greater than 100 mGy. On the other hand, genes with a constant modulation of their expression in all the tested doses were related to cellular respiration, ATP metabolic process and chromatin organization. These latter molecular mechanisms seem to be triggered at very low doses (5-25 mGy). In-silico promoter analysis seems to confirm the implication of transcription factors related to the pathways mentioned above.
2013-06-24 | GSE43151 | GEO
Project description:The aim of the present study is to analyze by microarray techniques how gene expression is modulated after exposure to low and very low doses of ionizing radiation, to evaluate if the pattern of gene expression shows dose dependence, and to search for putative regulatory mechanisms behind the observed gene-expression modifications.For this, whole blood samples from five healthy donors diluted in IMDM (1:10) were exposed at six doses between 5 and 500 mGy. Total RNA extraction from CD4+ lymphocytes was done at four different post-irradiation times (150, 300, 450 and 600 min). After mRNA amplification, aRNAs were hybridized on 4x44k DNA microarrays. The results indicated that up-regulation were twice than down-regulation. Surprisingly, the number of modulated genes doesnM-bM-^@M-^Yt seem to change drastically with dose, even at the lowest dose of 5 mGy. Clustering analysis revealed seven gene expression clusters with different dose dependence profiles. The functional analysis showed that the genes which increased their expression with the dose were related to p53 pathway and DNA damage response. This could be observed from 25 mGy, but becomes very clear at doses equal or greater than 100 mGy. On the other hand, genes with a constant modulation of their expression in all the tested doses were related to cellular respiration, ATP metabolic process and chromatin organization. These latter molecular mechanisms seem to be triggered at very low doses (5-25 mGy). In-silico promoter analysis seems to confirm the implication of transcription factors related to the pathways mentioned above. Human whole blood samples from 5 healthy donors were diluted in IMDM medium (1:10) and exposed to 500,100,50,25,10 and 5 mGy of Cobalt 60 gamma-rays. Gene expression alterations were measured 150, 300, 450 and 600 min post exposure after negative cell sorting of lymphocytes TCD4+ cells.
2013-06-24 | E-GEOD-43151 | biostudies-arrayexpress
Project description:Background: Polybrominated diphenyl ethers are a group of flame-retardant chemicals appearing increasingly in the environment. Their health effects and mechanisms of toxicity are poorly understood.Objectives: We screened for the sensitive effects and mechanisms of toxicity of 2,2 ,4,4 -tetra-bromodiphenyl ether (BDE-47) by analyzing the gene expression profile in rats exposed to doses comparable to human exposure.Methods: Wistar dams were exposed to vehicle or BDE-47 (0.002 and 0.2 mg/kg body weight) every fifth day from gestation day 15 to postnatal day 20 by injections to caudal vein. Total RNA was extracted from the livers of pups and hybridized to the whole-genome RNA expression micro-arrays. The list of genes 2-fold differentially expressed was exported to PANTHER and Ingenuity Systems for analysis of enriched ontology groups and molecular pathways.Results: Oxidoreductase and transferase protein families were enriched in exposed rats as were these biological process categories: carbohydrate metabolism; electron transport; and lipid, fatty acid, and steroid metabolism. Four signaling pathways (cascades of activation of drug-metabolizing enzymes) and 10 metabolic pathways were significantly enriched. Drug-metabolizing enzymes appear to be regulated by BDE-47 through an aryl hydrocarbon receptorindependent mechanism. Direct interaction with retinoid X receptor or its upstream cascade may be involved. The main metabolic effects consisted of activation of metabolic pathways: alpha- and omega-oxidation of fatty acids, glycolysis, and starch hydrolysis.Conclusions: Altered expression of genes involved in metabolic and signaling pathways and functions of the organism occurs after perinatal exposure of rat offspring to BDE-47 at doses relevant for the general population.
2010-01-13 | GSE19868 | GEO
Project description:Analysis of developmental toxicity of environmental toxin 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) at the level of gene expression. Dams of Wistar rats were exposed to environmentaly relevant doses of BDE-47 at gestation and lactation. Gene expression in total brains and frontal lobes was analysed in offspring on postnatal day 10.
2010-01-13 | GSE19867 | GEO
Project description:We have identified several nucleotide motifs (caug, cgggag=S2) that promote exosome sorting of miRNA in different cell types including brown adipocytes. In order to identify which proteins might recognize and bind to these motifs, we have performed co-precipitations of proteins binding biotinylated forms of miRNAs containing the aforementioned motifs or none - using streptavidin beads incubated with brown adipocytes cell lysates. We have included two types of controls: negative poly-A control and a scramble miRNA.
2021-01-30 | MSV000086780 | MassIVE
Project description:CD248 knockdown in in vitro differentiated adipocytes exposed to hypoxia
| PRJNA544327 | ENA
Project description:Omental adipose tissue explants were cultured for 24h in serum-free medium in the presence of vehicle (control medium) or macrophage (LPS) and T-cell (anti-CD3/28) stimulants (active medium). SGBS human preadipocytes were differentiated into adipocytes and then exposed to 25% v/v control or active medium.
2016-12-01 | GSE75710 | GEO