Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Whole blood RNA-seq of pediatric cases of natural Chikungunya infection

PROVIDER: PRJNA390289 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR5680260_1.fastq.gz	Fastqsanger.gz
	SRR5680260_2.fastq.gz	Fastqsanger.gz
	SRR5680270_1.fastq.gz	Fastqsanger.gz
	SRR5680270_2.fastq.gz	Fastqsanger.gz
	SRR5680280_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 344

Similar Datasets

Whole blood RNA-seq of pediatric cases of natural Dengue infection

Project description:Whole blood RNA-seq of pediatric cases of natural Dengue infection

| PRJNA590999 | ENA

Whole blood RNA-seq of pediatric cases of natural Chikungunya infection

Project description:The primary aim of this study was to characterize the transcriptomic changes between the acute and convalescent phase of natural Chikungunya infections in pediatric patients as observed in peripheral blood samples.

2018-06-13 | GSE99992 | GEO

Homo sapiens

Project description:Whole Blood Transcriptional Response to Pediatric Influenza Infection

| PRJNA140201 | ENA

N6-methyladenosine modification is not a general trait of positive-sense RNA viral genomes

Project description:Despite the nuclear localization of the m6A machinery, the genomes of multiple exclusively-cytoplasmic RNA viruses, such as chikungunya (CHIKV) and dengue (DENV), are reported to be extensively m6A-modified. However, these findings are mostly based on m6A-seq, an antibody-dependent technique with a high rate of false positives. Here, we addressed the presence of m6A in CHIKV and DENV RNAs. For this, we combined m6A-seq and the antibody-independent SELECT and nanopore direct RNA sequencing techniques with functional, molecular, and mutagenesis studies. Following this comprehensive analysis, we found no evidence of m6A modification in CHIKV or DENV transcripts. Furthermore, depletion of key components of the host m6A machinery did not affect CHIKV or DENV infection. Moreover, CHIKV or DENV infection had no effect on the m6A machinery’s localization. Our results challenge the prevailing notion that m6A modification is a general feature of cytoplasmic RNA viruses and underscore the importance of validating RNA modifications with orthogonal approaches.

2024-01-25 | MTBLS6978 | MetaboLights

Pathogenesis of chikungunya virus infection associated with fatal outcome

Project description:Chikungunya virus (CHIKV) is a mosquito-borne virus that causes acute, subacute, and chronic human diseases and can cause neurological complications and death. Here, we combined epidemiological, virological, histopathological, cytokine, molecular dynamics, metabolomics, proteomics, and genomic analyses to investigate viral and pathophysiologic factors that contribute to deaths caused by chikungunya (CHIK). Our results indicate that CHIK-deaths presented multiple organs infection, central nervous system damage, and exhibited significantly elevated serum levels of pro-inflammatory cytokines and chemokines compared to survivors. The histopathology, metabolite, and proteomic signatures of CHIK-deaths revealed hemodynamic disorders and dysregulated immune system response. CHIKV East-Central-South-African lineage caused fatal and survivor cases, and CHIKV crosses the blood–brain barrier without tight junction alterations. IFN-λ3 was highly expressed in fatal cases but did not present a direct antiviral effect on CHIKV replication in vitro. In summary, our results reveal insights to improve understanding of CHIK pathogenesis, especially the pathophysiology of fatal infections.

2024-06-16 | PXD043815 | Pride

Elucidation of the epitranscriptomic RNA modification landscape of chikungunya virus

Project description:The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected to a wide range of RNA modifications. In this study, we focused on the chikungunya virus (CHIKV) as a model (+) ssRNA virus to study the landscape of viral RNA modification in infected human cells. Among the 32 distinct RNA modifications analyzed by mass spectrometry, inosine was found enriched in the genomic CHIKV RNA. However, orthogonal validation by Illumina RNA-seq analyses did not identify any inosine modification along the CHIKV RNA genome. Moreover, CHIKV infection did not alter the expression of ADAR1 isoforms, the enzymes that catalyze the adenosine to inosine conversion. Together, this study highlights the importance of a multidisciplinary approach to assess the presence of RNAmodifications in viral RNA genomes.

2024-06-11 | MTBLS8697 | MetaboLights

Whole Blood Transcriptional Response to Pediatric Influenza Infection

Project description:The objective of this study was to identify the transcriptional siganture of influenza infection in a pediatric populationfor comparison with other disease datasets. Pediatric influenza patients (n=19) had whole blood samples collected into Tempus tubes upon presentation at local hospital with follow up confirmation of influenza infection. Age matched healthy controls were also collected (n=12) for microarray anlysis

2015-05-12 | E-GEOD-29366 | biostudies-arrayexpress

RNA-seq of whole blood from pediatric Sickle Cell Anemia (SCA) patients

Project description:RNA-seq of whole blood from pediatric Sickle Cell Anemia (SCA) patients

| PRJNA777971 | ENA

RNA-seq of whole blood from pediatric Sickle Cell Anemia (SCA) patients

Project description:RNA-seq of whole blood from pediatric Sickle Cell Anemia (SCA) patients

| PRJNA777972 | ENA

Homo sapiens whole blood infected with Candida spp. (here: Candida glabrata)

Project description:Homo sapiens fresh whole blood was infected with Candida glabrata. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Measurement of Candida glabrata gene expression.

2018-12-26 | GSE114175 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data