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Bacteria

ABSTRACT: Strong effects of plant species on stream bacterial communities via leachate from leaf litter

PROVIDER: PRJNA396217 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR5890910.fastq.gz	Fastqsanger.gz
	SRR5890920.fastq.gz	Fastqsanger.gz
	SRR5890930.fastq.gz	Fastqsanger.gz
	SRR5890901.fastq.gz	Fastqsanger.gz
	SRR5890911.fastq.gz	Fastqsanger.gz

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Project description:The effect of sucrose feeding on gene expression in Arabidopsis thaliana leaves was investigated using affymetrix ATH1 microarrays. For this, petioles of detached leaves were put in a solution containing either sucrose or sorbitol (control). Sugars were taken up into the leaf via the respiration stream for 13 hours. After that, leaves were frozen in liquid nitrogen and RNA was extracted for analysis. Experiment Overall Design: The effect of sucrose feeding on gene expression was investigated using affymetrix ATH1 microarrays. For this, petioles of detached leaves were put in a solution containing either sucrose or sorbitol (control). Sugars were taken up into the leaf via the respiration stream for 13 hours. After that, leaves were frozen in liquid nitrogen and RNA was extracted for analysis.

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Jackrel Lab Microcosm Experiment - DOM Concentrated Samples - Prelim Upload - Full Upload once results files available

Project description:We tested whether home field advantage at inter- and intra-specific levels alters microbial carbon transformations, using a multi-factorial design with microcosms of freshwater submerged leaf litter from two species (Alder and Hemlock) exposed to 'home' or 'away' communities of microbes isolated from decomposing leaf litter, as well as controls with no microbial community added. For all 'home', 'away' and control conditions for each species we also had high or low oxygen treatments. Samples were taken at day 0, 154, 257 and 354 and extracted with solid phase columns and methanol to provide extracted metabolites.

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