Project description:Slc39a8 KO mouse embryo hearts exhibit ventricle noncompaction phenotype which becomes evident at E12.5. The goal of this experiment is to identify genes that are differentially expressed between Slc39a8 KO and WT, which may be underlying the phenotype.
Project description:Using RNAseq to identify differentially expressed transcripts between CBFB wild type (WT) and knockout (KO) or between RUNX1 wild type (WT) and knockout (KO) MCF10A cells.
Project description:To investigate the role of NBCe1-B in the heart we performed RNAseq experiments to examine genome-wide transcriptional changes in female NBCe1-B/C knockout (KO) mice compared to female wild-type (WT) mice.
Project description:Peroxisomes are versatile single membrane-enclosed cytoplasmic organelles, involved in reactive oxygen species (ROS) and lipid metabolism and diverse other metabolic processes. Peroxisomal disorders result from mutations in Pex genes-encoded proteins named peroxins (PEX proteins) and single peroxisomal enzyme deficiencies. The PEX11 protein family (α, β, and γ isoforms) plays an important role in peroxisomal proliferation and fission. However, their specific functions and the metabolic impact caused by their deficiencies have not been precisely characterized. To understand the systemic molecular alterations caused by peroxisomal defects, here we utilized untreated peroxisomal biogenesis factor 11α knockout (Pex11α KO) mouse model and performed serial relative-quantitative lipidomic, metabolomic, and proteomic analyses of serum, liver, and heart tissue homogenates. We demonstrated significant specific changes in the abundances of multiple lipid species, polar metabolites, and proteins and dysregulated metabolic pathways in distinct biological specimens of the Pex11α KO adult mice in comparison to the wild type (WT) controls. Overall, the present study reports comprehensive semi-quantitative molecular omics information of the Pex11α KO mice, which might serve in the future as a reference for a better understanding of the roles of Pex11α and underlying pathophysiological mechanisms of peroxisomal biogenesis disorders.
Project description:Metabolomics data from E. Coli Nissle (wild type, WT) and 3 E. Coli Nissle knockout (KO) strains grown in M-9 media and enriched M-9 media.
Project description:We investigated the effect of Bola3 in brown fat mitochondria. From Bola3 knockout (KO) mice and wild type (WT) control, we isolated mitochondria from brown fat for proteome profiling. We applied 250 ng peptides for LC/MS/MS analysis using label-free quantification coupled match-between-run approach.
Project description:Untargeted metabolomics from heart ventricular tissues of wild-type (WT) and cardiomyocyte-specific conditional NAMPT knockout (KO) mice, harvested at 8-weeks post-NAMPT deletion. Mass spectrometer was operated in both negative (NEG, 70 to 1000 m/z) and positive (POS, 50 to 117.9 m/z) ion mode with the HILIC 25-minute method for the detection of metabolites.
Project description:We used quantitative proteomics to characterize the p300-regulated lysine crotonylome in wild type (WT) and p300 knockout (KO) cells.
