Project description:The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNASeq method by performing next-generation sequencing on single glomus cellderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB.
Project description:The carotid body (CB) is a major arterial chemoreceptor containing glomus cells whose activities are regulated by changes in arterial blood contents including oxygen. We employed the single cell RNAÂSeq method by performing next-generation sequencing on single glomus cellÂderived cDNAs to eliminate contamination of genes derived from other cell types present in the CB. This study has generated a CB expression profile containing novel glomus cell specific genes. The wealth of information provided through this study offers a valuable foundation for identifying molecules functioning in the CB. 8 single CB glomus cells that were confirmed expressing tyrosine hydroxylase (Th), ubiquitin carboxyl-terminal esterase L1 (Uchl1) and potassium channel subfamily K member 3 (Kcnk3) were harvested from P4-5 wild type mice and subjected to single-cell RNA-Seq
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from uninfected and infected samples at 3 time points (3, 10 and 20 days). Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans
Project description:The carotid body is a peripheral chemoreceptor organ containing a neurogenic niche with stem cells and immature neuronal cells that convert into new neurons (glomus cells) upon activation by hypoxia. We have used microarray to compare the gene expression programs of glomus cells and neuroblasts, both in normoxic and hypoxic (3 days) conditions, to highlight the molecular mechanisms underlying fast neurogenesis