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Streptococcus mutans YidC2: regulation and effects on the transcriptome

ABSTRACT: Streptococcus mutans YidC2: regulation and effects on the transcriptome

PROVIDER: PRJNA415071 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6192770.fastq.gz	Fastqsanger.gz
	SRR6192780.fastq.gz	Fastqsanger.gz
	SRR6192790.fastq.gz	Fastqsanger.gz
	SRR6192771.fastq.gz	Fastqsanger.gz
	SRR6192781.fastq.gz	Fastqsanger.gz

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Interactions with Commensal or Non-commensal Streptococci Alter Streptococcus mutans Transcriptome and Behaviors

Project description:RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture, in coculture with Streptococcus gordonii DL1, Streptococcus sanguinis SK36 or Streptococcus oralis 34, and in a quadculture containing all four species. Individual cultures of commensal species Streptococcus gordonii DL1, Streptococcus sanguinis SK36 and Streptococcus oralis 34 were sequenced as well. This revealed a common transcriptome pattern in S. mutans when grown in mixed-species culture, indepenedent of the species identity that S. mutans was cultured with. Additionally, transcriptome changes in the commensal species could also be determined when undergoing competition from S. mutans. RNA-Seq was used to compare the transcriptome of Streptococcus mutans UA159 during growth alone in monoculture or in coculture with Streptococcus sobrinus NIDR 6715, Lactobacillus casei ATCC 4646 or Corynebacterium matruchotii ATCC 14266. These data were compared to previous coculture and quadculture RNA-Seq data with commensal streptococci (GSE209925). These data confirmed a common transcriptome pattern in S. mutans when grown in mixed-species culture with commensal streptococci that is not present with non-commensal streptococci, indepenedent of the species identity that S. mutans was cultured with.

2022-08-03 | GSE209925 | GEO

ΔfruB effects on the transcriptome of Streptococcus mutans probed by RNA-seq

Project description:Streptococcus mutans, the primary etiologic agent of human dental caries, and a variety of oral Streptococcus and Actinomyces spp. synthesize high molecular mass homopolymers of fructose (fructans) with predominantly β2,1- (inulins) or β2,6-linkages (levans). The ability of S. mutans to degrade fructans contributes to the severity of dental caries. The extracellular product of fruA of S. mutans is an exo-β-D-fructofuranosidase that releases fructose from levan and inulin. Located 70 bp downstream of fruA, fruB encodes a member of the glycoside hydrolase family 32, but the function of FruB has not been established.In this study, the transcriptomic analysis of UA159 and a fruB mutant grown on 0.2% levan revealed differential expression of genes encoding ABC transporters, transcriptional regulators and genes involved in growth and stress tolerance. The results provide new insights into the mechanisms of action by which FruB utilizes homo-polymers of fructose, specifically levan. The findings significantly enhance our understanding of the genetics and physiology of this cariogenic pathogen S. mutans.

2022-04-15 | GSE200727 | GEO

Transcriptome analysis of Streptococcus mutans quorum sensing-induced cells

Project description:In the human pathogen Streptococcus mutans, the canonical peptide-based quorum sensing system is an inducible DNA repair system that is pivotal for bacterial survival. Previous work showed that the CSP signaling peptide is a stress-signaling alarmone that controls different stress-induced phenotypes. In this study, we exposed S. mutans to the CSP pheromone to mimic DNA damage conditions. Transcriptome analysis was then performed to evaluate the differential gene expression between the normal stationary phase cells and the CSP-induced stationary phase cells. The data obtained contribute to the understanding of the CSP-induced phenotypes in S. mutans.

2024-04-28 | GSE264670 | GEO

Arginine-specific effects on the transcriptome of Streptococcus mutans probed by RNA-seq

Project description:Streptococcus mutans is a common constituent of oral biofilms and a primary etiologic agent of human dental caries. The bacteria associated with dental caries have a potent ability to produce organic acids from dietary carbohydrates and to grow and metabolize in acidic conditions. In this study, we observed supplementation with 1.5% arginine (final concentration) had inhibitory effects on the growth of S. mutans in complex and chemically defined media, particularly when cells were exposed to acid or oxidative stress. Deep-sequencing of RNA (RNA-Seq) comparing the transcriptomes of S. mutans growing in a chemically defined medium with and without 1.5% arginine in neutral and acidic pH conditions and under oxidative stress conditions revealed interesting results. The results provide new insights into the mechanisms of action by which arginine inhibits dental caries through direct adverse effects on multiple virulence-related properties of the most common human dental caries pathogen. The findings significantly enhance our understanding of the genetics and physiology of this cariogenic pathogen.

2017-05-20 | GSE98020 | GEO

Streptococcus mutans UA159 control vs. Streptococcus mutans UA159 supplemented with 20 uM 4,5-dihydroxy-2,3-pentadione (DPD) at 3 different pH values

Project description:Transcriptional profiling of early logarithmic phase culture (O.D=0.2-0.3) of Streptococcus mutans UA159 comparing control of untreated Streptococcus mutans UA159 bacteria with Streptococcus mutans UA159 bacteria spplemented with 20µM synthetic DPD (pre-AI-2) which regulates gene expression via AI-2 quorum sensing system.Three compairisons were performed at pHs of 7,6 and 5.

2010-07-29 | GSE23159 | GEO

Transcriptome analysis of Streptococcus mutans biofilms in response to cranberry proanthocyanidins

Project description:The influence of cranberry proanthocyanidins on the transcriptomic responses of Streptococcus mutans during biofilm formation was investigated.

2012-08-22 | GSE40172 | GEO

Streptococcus mutans YidC2: regulation and effects on the transcriptome

Project description:Differential gene expression was determined between wild type Streptococcus mutan and a yidC1 or yidC2 mutant using whole transcriptome RNA sequence. A significantly greater number of genes were differentially expressed (DE) in the yidC2 mutant (320 DE genes) compared to wildtype, while the yidC1 mutant had only 21 DE genes when compared to wildtype.

2018-10-19 | GSE105254 | GEO

The impacts of the Spx regulator and H2O2 stress on the transcriptome of Streptococcus mutans

Project description:As a resident of the oral cavity, Streptococcus mutans must withstand a variety of stresses, including oxidative stress. Previously, we reported the identification of two Spx homologues (SpxA1 and SpxA2) in Streptococcus mutans that appear critical in the ability of the microbe to tolerate oxidative stresses; SpxA1 was shown to play a primary role in activation of detoxification strategies whereas SpxA2 served as a secondary activator. Here, we used RNA deep sequencing (RNA-Seq) to examine the transcriptional changes associated with H2O2 stress in the parent S. mutans strain UA159 and to compare the peroxide-stress transcriptome of UA159 with its Δspx derivatives. The transcriptome data confirmed the relationship between SpxA1 and genes typically associated with oxidative stress responses, but also identified new genes and metabolic pathways that are activated during peroxide stress, also in an SpxA1-dependent manner.

2017-05-08 | GSE98526 | GEO

Transcriptome analysis of Streptococcus mutans biofilms in response to starch and sucrose.

Project description:Transcriptional profiling to investigate the response of Streptococcus mutans biofilms to starch and sucrose at distinct stages of biofilm development.

2010-05-15 | GSE21831 | GEO

Sucrose- and fructose-specific effects on the transcriptome of Streptococcus mutans probed by RNA-Seq

Project description:For Streptococcus mutans, the major etiologic agent of human dental caries, sucrose is the carbohydrate that contributes in the most significant manner to establishment, persistence and virulence of the organism. However, because this organism produces multiple extracellular sucrolytic enzymes that can release hexoses from sucrose, it has not been possible to study the specific effects of sucrose transport and metabolism on gene expression in the absence of carbohydrates that by themselves can elicit catabolite repression and induce expression of multiple genes. Employing RNA deep sequencing (RNA-Seq) technology and mutants that lacked particular sucrose-metabolizing enzymes, we compared the transcriptomes of S. mutans growing on glucose, fructose or sucrose as the sole carbohydrate. The results provide a variety of new insights into the impact of sucrose transport and metabolism by S. mutans, including the likely expulsion of fructose after sucrose internalization and hydrolysis, and identified a set of genes that are differentially regulated by sucrose versus fructose. The findings significantly enhance our understanding of the genetics and physiology of this cariogenic pathogen.

2015-11-12 | GSE74939 | GEO

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