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Comparative transcriptome analysis of murine macrophages infected with Brucella suis vaccine strain S2 and the virulent Brucella suis strain 1330

ABSTRACT: Comparative transcriptome analysis of murine macrophages infected with Brucella suis vaccine strain S2 and the virulent Brucella suis strain 1330

PROVIDER: PRJNA415479 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6204820_1.fastq.gz	Fastqsanger.gz
	SRR6204820_2.fastq.gz	Fastqsanger.gz
	SRR6204821_1.fastq.gz	Fastqsanger.gz
	SRR6204821_2.fastq.gz	Fastqsanger.gz
	SRR6204822_1.fastq.gz	Fastqsanger.gz

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Comparative transcriptome analysis of murine macrophages infected with Brucella suis vaccine strain S2 and the virulent Brucella suis strain 1330

Project description:Brucellosis is one of the most common zoonotic epidemics worldwide. Vaccination against Brucellosis is an important control strategy to prevent the disease in many high-prevalence regions. At present, Brucella vaccine strain S2 is the most widely used vaccine in China. In this study, to uncover the related mechanisms underlie virulence attenuation of S2, we characterized the transcriptional profile of S2 and 1330 infected macrophages by transcriptome analysis. The results revealed that expressions of 440 genes were significantly different between macrophages infected by 1330 and S2. Data analysis showed that in the gene ontology term, the different expressed genes involved in innate immune response, phagoctyosis, recognition, and inflammatory response were significantly enriched. Pathway enrichment analysis indicated that the genes involved in transcriptional misregulation in cancer, staphylococcus aureus infection pathways and NF-kappa B signaling pathway were significantly affected. To reveal the molecular mechanisms related to different expression profiles of infected macrophages, the transcription levels of the different genes between the two bacterial genomes were also detected. In total, the transcription of 29 different genes was significantly changed in either culture medium or infected microphages. The results of current study can be conducive to the promotion of better understanding of the related mechanisms underlie virulence attenuation of S2 and interactions between host cells and brucella strains.

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Project description:To explore the role of Brucella BI-1 in Brucella suis S2, we constructed the Brucella BI-1 deletion mutant strain and its complementary strain. We then determined the effect of Brucella BI-1 deletion on the physiological characteristics of Brucella suis S2 and revealed them via integrated transcriptomic and proteomic analyses. Brucella BI-1 deletion altered the membrane properties of Brucella suis S2 and decreased its resistance to acidic pH, H2O2, polymyxin B, and lincomycin. Additionally, deleting Brucella BI-1 led to defective growth, cell division, and viability in Brucella suis S2. In conclusion, our results revealed that Brucella BI-1 is a bacterial cytoprotective protein involved in membrane homeostasis, cell division, and stress resistance in Brucella suis S2.

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